Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1975 Aug;12(2):440–442. doi: 10.1128/iai.12.2.440-442.1975

Measurement of biological activities of purified and crude enterotoxin of Clostridium perfringens.

L Nilo
PMCID: PMC415302  PMID: 168155

Abstract

Enterotoxin of Clostridium perfringens was assayed and compared with toxicity in mice and erythemal activity in guinea pigs. Conversion factors were used to express these biological activities of crude enterotoxin in terms of weight of pure enterotoxin protein. One microgram of enterotoxin was equivalent to 3.41 erythema units and to 0.68 mouse median lethal dose.

Full text

PDF
440

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  2. Duncan C. L., Somers E. B. Quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion. Appl Microbiol. 1972 Nov;24(5):801–804. doi: 10.1128/am.24.5.801-804.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Genigeorgis C., Sakaguchi G., Riemann H. Assay methods for Clostridium perfringens type A enterotoxin. Appl Microbiol. 1973 Jul;26(1):111–115. doi: 10.1128/am.26.1.111-115.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hauschild A. H. Erythemal activity of the cellular enteropathogenic factor of Clostridium perfringens type A. Can J Microbiol. 1970 Aug;16(8):651–654. doi: 10.1139/m70-112. [DOI] [PubMed] [Google Scholar]
  5. Hauschild A. H., Hilsheimer R., Martin W. G. Improved purification and further characterization of Clostridium perfringens type A enterotoxin. Can J Microbiol. 1973 Nov;19(11):1379–1382. doi: 10.1139/m73-222. [DOI] [PubMed] [Google Scholar]
  6. Hauschild A. H., Hilsheimer R. Purification and characteristics of the enterotoxin of Clostridium perfringens type A. Can J Microbiol. 1971 Nov;17(11):1425–1433. doi: 10.1139/m71-227. [DOI] [PubMed] [Google Scholar]
  7. Hauschild A. H., Niilo L., Dorward W. J. The role of enterotoxin in Clostridium perfringens type A enteritis. Can J Microbiol. 1971 Jul;17(7):987–991. doi: 10.1139/m71-156. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Niilo L., Dorward W. J. The effect of enterotoxigenic Clostridium welchii (perfringens) type A on the bovine intestine. Res Vet Sci. 1971 Jul;12(4):376–378. [PubMed] [Google Scholar]
  10. Niilo L. Effect on calves of the intravenous injection of the enterotoxin of Clostridium welchii type A. J Comp Pathol. 1973 Apr;83(2):265–269. doi: 10.1016/0021-9975(73)90051-0. [DOI] [PubMed] [Google Scholar]
  11. Niilo L. Fluid secretory response of bovine Thiry jejunal fistula to enterotoxin of Clostridium perfringens. Infect Immun. 1973 Jan;7(1):1–4. doi: 10.1128/iai.7.1.1-4.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Niilo L., Hauschild A. H., Dorward W. J. Immunization of sheep against experimental Clostridium perfringens type A enteritis. Can J Microbiol. 1971 Mar;17(3):391–395. doi: 10.1139/m71-065. [DOI] [PubMed] [Google Scholar]
  13. Niilo L. Mechanism of Action of the Enteropathogenic Factor of Clostridium perfringens Type A. Infect Immun. 1971 Jan;3(1):100–106. doi: 10.1128/iai.3.1.100-106.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Niilo L. Response of ligated intestinal loops in chickens to the enterotoxin of Clostridium perfringens. Appl Microbiol. 1974 Nov;28(5):889–891. doi: 10.1128/am.28.5.889-891.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sakaguchi G., Uemura T., Riemann H. P. Simplified method for purification of Clostridium perfringens type A enterotoxin. Appl Microbiol. 1973 Nov;26(5):762–767. doi: 10.1128/am.26.5.762-767.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stark R. L., Duncan C. L. Biological characteristics of Clostridium perfringens type A enterotoxin. Infect Immun. 1971 Aug;4(2):89–96. doi: 10.1128/iai.4.2.89-96.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stark R. L., Duncan C. L. Purification and biochemical properties of Clostridium perfringens type A enterotoxin. Infect Immun. 1972 Nov;6(5):662–673. doi: 10.1128/iai.6.5.662-673.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES