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. 1975 Sep;12(3):536–543. doi: 10.1128/iai.12.3.536-543.1975

Affinity chromatography purification of Clostridium perfringens enterotoxin.

V N Scott, C L Duncan
PMCID: PMC415320  PMID: 170205

Abstract

Anti-enterotoxin immunoglobulins immobilized on CH-Sepharose or CNBr-Sepharose were used for affinity chromatography purification of Clostridium perfringens enterotoxin. Cell extracts containing enterotoxin or partially purified toxin preparations were applied to the column and nonspecifically-bound protein was eluted. NaOH was used to elute specifically bound toxin. The purity of enterotoxin purified by Sephadex G-100 chromatography followed by affinity chromatography appears similar to toxin highly purified by conventional means. The procedure can be used successfully for the rapid (less than 2 h) purification of small amounts of enterotoxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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