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. 1975 Sep;12(3):630–637. doi: 10.1128/iai.12.3.630-637.1975

Scanning electron microscopic study of virulent and avirulent colonies of Neisseria gonorrhoeae.

T Elmros, P Hörstedt, B Winblad
PMCID: PMC415334  PMID: 809360

Abstract

A preparation procedure for scanning electron microscopy was developed by which gononcoccal colonies can be studied directly on the agar surface. After glutaraldehyde fixation directly in petri dishes, small agar pieces were cut out and dehydrated stepwise in increasing concentrations of ethanol. The blocks were thereafter transferred to a critical-point drying apparatus, via steps of increased gradients up to 100% amyl acetate. By this method five different gonococcal colony types could be distinguished analogous to light microscopic observations made by others. At higher magnifications an abundance of intercellular strands was found between the cells in virulent type 1 and 2 colonies, but not in the avirulent types 3 through 5. These strands seemed to anchor the cells to each other and to the agar surface. The presence of such structures probably explains the highly convex surface of virulent colonies and explains why colonies of avirulent strains exhibit a radial extension and a flat upper surface. The nature of these filamentous intercellular strands is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Afrikian E. G., St Julian G., Bulla L. A., Jr Scanning electron microscopy of bacterial colonies. Appl Microbiol. 1973 Dec;26(6):934–937. doi: 10.1128/am.26.6.934-937.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bibel D. J., Lawson J. W. A simple method for scanning electron microscopy of L-form colonies grown on agar. Can J Microbiol. 1971 Jun;17(6):822–823. doi: 10.1139/m71-131. [DOI] [PubMed] [Google Scholar]
  3. Bibel D. J., Lawson J. W. Scanning electron microscopy of L-phase streptococci. I. Development of techniques. J Microsc. 1972 Jun;95(3):453–458. doi: 10.1111/j.1365-2818.1972.tb01048.x. [DOI] [PubMed] [Google Scholar]
  4. Buchanan T. M., Gotschlich E. C. Studies on gonococcus infection. 3. Correlation of gonococcal colony morphology with infectivity for the chick embryo. J Exp Med. 1973 Jan 1;137(1):196–200. doi: 10.1084/jem.137.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jephcott A. E., Reyn A., Birch-Andersen A. Neisseria gonorrhoeae 3. Demonstration of presumed appendages to cells from different colony types. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(3):437–439. doi: 10.1111/j.1699-0463.1971.tb00086.x. [DOI] [PubMed] [Google Scholar]
  6. Jephcott A. E., Reyn A. Neisseria gonorrhoeae. Colony variation I. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(5):609–614. doi: 10.1111/j.1699-0463.1971.tb00088.x. [DOI] [PubMed] [Google Scholar]
  7. Johnston K. H., Gotschlich E. C. Isolation and characterization of the outer membrane of Neisseria gonorrhoeae. J Bacteriol. 1974 Jul;119(1):250–257. doi: 10.1128/jb.119.1.250-257.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kellogg D. S., Jr, Cohen I. R., Norins L. C., Schroeter A. L., Reising G. Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro. J Bacteriol. 1968 Sep;96(3):596–605. doi: 10.1128/jb.96.3.596-605.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kraus S. J., Glassman L. H. Scanning electron microscope study of Neisseria gonorrhoeae. Appl Microbiol. 1974 Mar;27(3):584–592. doi: 10.1128/am.27.3.584-592.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lucas C. T., Chandler F., Jr, Martin J. E., Jr, Schmale J. D. Transfer of gonococcal urethritis from man to chimpanzee. An animal model for gonorrhea. JAMA. 1971 Jun 7;216(10):1612–1614. [PubMed] [Google Scholar]
  12. Ovcinnikov N. M., Delektorskij V. V. Electron microscope studies of gonococci in the urethral secretions of patients with gonorrhoea. Br J Vener Dis. 1971 Dec;47(6):419–439. doi: 10.1136/sti.47.6.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pirt S. J. A kinetic study of the mode of growth of surface colonies of bacteria and fungi. J Gen Microbiol. 1967 May;47(2):181–197. doi: 10.1099/00221287-47-2-181. [DOI] [PubMed] [Google Scholar]
  14. Reyn A., Jephcott A. E., Ravn H. Neisseria gonorrhoeae. Colony variation II. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(3):435–436. doi: 10.1111/j.1699-0463.1971.tb00085.x. [DOI] [PubMed] [Google Scholar]
  15. Rohrschneider I., Schinko I., Wetzstein R. Vergleichende rasterelektronenmikroskopische. Untersuchungen an der Froschlunge nach Lufttrocknung und nach Anwendung der "critical point"--Methode. Mikroskopie. 1973 May;29(3):116–121. [PubMed] [Google Scholar]
  16. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sparling P. F., Yobs A. R. Colonial morphology of Neisseria gonorrhoeae isolated from males and females. J Bacteriol. 1967 Jan;93(1):513–513. doi: 10.1128/jb.93.1.513-.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Swanson J., Kraus S. J., Gotschlich E. C. Studies on gonococcus infection. I. Pili and zones of adhesion: their relation to gonococcal growth patterns. J Exp Med. 1971 Oct 1;134(4):886–906. doi: 10.1084/jem.134.4.886. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Swanson J. Studies on gonococcus infection. II. Freeze-fracture, freeze-etch studies on gonocci. J Exp Med. 1972 Nov 1;136(5):1258–1271. doi: 10.1084/jem.136.5.1258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ward M. E., Watt P. J. Adherence of Neisseria gonorrhoeae to urethral mucosal cells: an electron-microscopic study of human gonorrhea. J Infect Dis. 1972 Dec;126(6):601–605. doi: 10.1093/infdis/126.6.601. [DOI] [PubMed] [Google Scholar]
  21. Whittaker D. K., Drucker D. B. Scanning electron microscopy of intact colonies of microorganisms. J Bacteriol. 1970 Nov;104(2):902–909. doi: 10.1128/jb.104.2.902-909.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zollinger W. D., Kasper D. L., Veltri B. J., Artenstein M. S. Isolation and characterization of a native cell wall complex from Neisseria meningitidis. Infect Immun. 1972 Nov;6(5):835–851. doi: 10.1128/iai.6.5.835-851.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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