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. 1975 Nov;12(5):999–1005. doi: 10.1128/iai.12.5.999-1005.1975

Murine model for study of cell-mediated immunity: protection against death from fully virulent Francisella tularensis infection.

H T Eigelsbach, D H Hunter, W A Janssen, H G Dangerfield, S G Rabinowitz
PMCID: PMC415388  PMID: 1193736

Abstract

To assess cell-mediated immunity in terms of host protection, an experimental model was developed in which passively transferred spleen cells from immunized AKR/J mice enabled nonimmume syngeneic recipients to survive an otherwise fatal infection with fully virulent Francisella tularensis. Donor immunization was achieved by administering live attenuted tularemia vaccine and, subsequently, the virulent streptomycin-sensitive SCHU S4 strain of F. tularensis. At selected intervals after immunization, donor spleen cells were transferred to streptomycin-treated recipients challenged subcutaneously, intravenously, or intraperitoneally with 25 to 50 minimal lethal doses of virulent streptomycin-resistant F. tularensis SCHU S5. The protection afforded by immune spleen cells was maximal (essentially 100%) 12 days after the SCHU S4 secondary immunization.

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Selected References

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  1. ALLEN W. P. Immunity against tularemia: passive protection of mice by transfer of immune tissues. J Exp Med. 1962 Feb 1;115:411–420. doi: 10.1084/jem.115.2.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blanden R. V., Lefford M. J., Mackaness G. B. The host response to Calmette-Guérin bacillus infection in mice. J Exp Med. 1969 May 1;129(5):1079–1107. doi: 10.1084/jem.129.5.1079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blanden R. V., Mackaness G. B., Collins F. M. Mechanisms of acquired resistance in mouse typhoid. J Exp Med. 1966 Oct 1;124(4):585–600. doi: 10.1084/jem.124.4.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Claflin J. L., Larson C. L. Infection-immunity in tularemia: specificity of cellular immunity. Infect Immun. 1972 Mar;5(3):311–318. doi: 10.1128/iai.5.3.311-318.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collins F. M. Immunogenicity of living and heat-killed Salmonella pullorum vaccines. Infect Immun. 1973 May;7(5):735–742. doi: 10.1128/iai.7.5.735-742.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Collins F. M., Mackaness G. B. The relationship of delayed hypersensitivity to acquired antituberculous immunity. I. Tuberculin sensitivity and resistance to reinfection in BCG-vaccinated mice. Cell Immunol. 1970 Sep;1(3):253–265. doi: 10.1016/0008-8749(70)90047-x. [DOI] [PubMed] [Google Scholar]
  7. Collins F. M. Vaccines and cell-mediated immunity. Bacteriol Rev. 1974 Dec;38(4):371–402. doi: 10.1128/br.38.4.371-402.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EIGELSBACH H. T., BRAUN W., HERRING R. D. Studies on the variation of Bacterium tularense. J Bacteriol. 1951 May;61(5):557–569. doi: 10.1128/jb.61.5.557-569.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. EIGELSBACH H. T., DOWNS C. M. Prophylactic effectiveness of live and killed tularemia vaccines. I. Production of vaccine and evaluation in the white mouse and guinea pig. J Immunol. 1961 Oct;87:415–425. [PubMed] [Google Scholar]
  10. Eigelsbach H. T., Hornick R. B., Tulis J. J. Recent studies on live tularemia vaccine. Med Ann Dist Columbia. 1967 May;36(5):282–286. [PubMed] [Google Scholar]
  11. Hornick R. B., Eigelsbach H. T. Aerogenic immunization of man with live Tularemia vaccine. Bacteriol Rev. 1966 Sep;30(3):532–538. doi: 10.1128/br.30.3.532-538.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JENKIN C. R., ROWLEY D. BASIS FOR IMMUNITY TO TYPHOID IN MICE AND THE QUESTION OF "CELLULAR IMMUNITY". Bacteriol Rev. 1963 Dec;27:391–404. doi: 10.1128/br.27.4.391-404.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kostiala A. A., McGregor D. D., Logie P. S. Tularaemia in the rat. I. The cellular basis on host resistance to infection. Immunology. 1975 May;28(5):855–869. [PMC free article] [PubMed] [Google Scholar]
  14. MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MACKANESS G. B. THE IMMUNOLOGICAL BASIS OF ACQUIRED CELLULAR RESISTANCE. J Exp Med. 1964 Jul 1;120:105–120. doi: 10.1084/jem.120.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mackaness G. B., Hill W. C. The effect of anti-lymphocyte globulin on cell-mediated reistance to infection. J Exp Med. 1969 May 1;129(5):993–1012. doi: 10.1084/jem.129.5.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mackaness G. B. Resistance to intracellular infection. J Infect Dis. 1971 Apr;123(4):439–445. doi: 10.1093/infdis/123.4.439. [DOI] [PubMed] [Google Scholar]
  18. Mackaness G. B. The influence of immunologically committed lymphoid cells on macrophage activity in vivo. J Exp Med. 1969 May 1;129(5):973–992. doi: 10.1084/jem.129.5.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. McGregor D. D., Koster F. T., Mackaness G. B. The short lived small lymphocyte as a mediator of cellular immunity. Nature. 1970 Nov 28;228(5274):855–856. doi: 10.1038/228855a0. [DOI] [PubMed] [Google Scholar]
  20. OVERHOLT E. L., TIGERTT W. D., KADULL P. J., WARD M. K., CHARKES N. D., RENE R. M., SALZMAN T. E., STEPHENS M. An analysis of forty-two cases of laboratory-acquired tularemia. Treatment with broad spectrum antibiotics. Am J Med. 1961 May;30:785–806. doi: 10.1016/0002-9343(61)90214-5. [DOI] [PubMed] [Google Scholar]
  21. Robson H. G., Vas S. I. Resistance of inbred mice to Salmonella typhimurium. J Infect Dis. 1972 Oct;126(4):378–386. doi: 10.1093/infdis/126.4.378. [DOI] [PubMed] [Google Scholar]
  22. SASLAW S., EIGELSBACH H. T., PRIOR J. A., WILSON H. E., CARHART S. Tularemia vaccine study. II. Respiratory challenge. Arch Intern Med. 1961 May;107:702–714. doi: 10.1001/archinte.1961.03620050068007. [DOI] [PubMed] [Google Scholar]
  23. SASLAW S., EIGELSBACH H. T., WILSON H. E., PRIOR J. A., CARHART S. Tularemia vaccine study. I. Intracutaneous challenge. Arch Intern Med. 1961 May;107:689–701. doi: 10.1001/archinte.1961.03620050055006. [DOI] [PubMed] [Google Scholar]
  24. SEVER J. L. Passive transfer of resistance to tuberculosis through use of monocytes. Proc Soc Exp Biol Med. 1960 Feb;103:326–329. doi: 10.3181/00379727-103-25506. [DOI] [PubMed] [Google Scholar]
  25. SUTER E., RAMSEIAR H. CELLULAR REACTIONS IN INFECTION. Adv Immunol. 1964;27:117–173. doi: 10.1016/s0065-2776(08)60707-5. [DOI] [PubMed] [Google Scholar]
  26. Sawyer W. D., Dangerfield H. G., Hogge A. L., Crozier D. Antibiotic prophylaxis and therapy of airborne tularemia. Bacteriol Rev. 1966 Sep;30(3):542–550. doi: 10.1128/br.30.3.542-550.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. THORPE B. D., MARCUS S. PHAGOCYTOSIS AND INTRACELLULAR FATE OF PASTEURELLA TULARENSIS. 3. IN VIVO STUDIES WITH PASSIVELY TRANSFERRED CELLS AND SERA. J Immunol. 1965 Apr;94:578–585. [PubMed] [Google Scholar]
  28. Zinkernagel R. M., Blanden R. V., Langman R. E. Early appearance of sensitized lymphocytes in mice infected with Listeria monocytogenes. J Immunol. 1974 Feb;112(2):496–501. [PubMed] [Google Scholar]

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