Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1975 Nov;12(5):1012–1017. doi: 10.1128/iai.12.5.1012-1017.1975

Murine cytomegalovirus: induction of and sensitivity to interferon in vitro.

H K Oie, J M Easton, D V Ablashi, S Baron
PMCID: PMC415390  PMID: 172448

Abstract

Moderate amounts of viral inhibitor were produced by mouse embryo (ME) cultures infected with two strains of plaque-purified murine cytomegalovirus (MCV). This inhibitor was shown to be interferon, based on the possession of similar properties. The growth studies of MCV in ME cells showed that interferon was produced as early as 4 h after infection, infectious virus was produced between 12 to 16 h, and cytopathic effect was produced between 16 to 18 h. Since MCV-induced interferon production and the subsequent development of antiviral state occurred early, the long eclipse period may be due to an interferon-mediated delay of virus replication. Pretreatment of ME cells with varying concentrations of interferon before infection with MCV did not result in increased interferon production, but at high pretreatment doses a slight inhibitory effect on interferon production was observed. In vitro sensitivity studies showed that small doses of MCV were highly sensitive to the antiviral action of interferon, but higher viral doses proved to be markedly resistant. Although the available evidence does not permit a definitive interpretation of the mechanism by which MCV may show differing sensitivities to interferon action, the presence of a small interferon-resistant fraction of virus-infected cells may account for the observations.

Full text

PDF
1012

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ablashi D. V., Martos L. M., Gilden R. V., Hampar B. Preparation of rabbit immune serum with neutralizing activity against a simian cytomegalovirus (SA6). J Immunol. 1969 Jan;102(1):263–265. [PubMed] [Google Scholar]
  2. BABLANIAN R., EGGERS H. J., TAMM I. STUDIES ON THE MECHANISM OF POLIOVIRUS-INDUCED CELL DAMAGE. I. THE RELATION BETWEEN POLIOVIRUS,-INDUCED METABOLIC AND MORPHOLOGICAL ALTERATIONS IN CULTURED CELLS. Virology. 1965 May;26:100–113. doi: 10.1016/0042-6822(65)90030-9. [DOI] [PubMed] [Google Scholar]
  3. BARON S., BUCKLER C. E. CIRCULATING INTERFERON IN MICE AFTER INTRAVENOUS INJECTION OF VIRUS. Science. 1963 Sep 13;141(3585):1061–1063. doi: 10.1126/science.141.3585.1061. [DOI] [PubMed] [Google Scholar]
  4. Baron S., Buckler C. E., Friedman R. M., McCloskey R. V. Role of interferon during viremia. II. Protective action of circulating interferon. J Immunol. 1966 Jan;96(1):17–24. [PubMed] [Google Scholar]
  5. CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
  6. Catalano L. W., Jr, Baron S. Protection against herpes virus and encephalomyocarditis virus encephalitis with a double-stranded RNA inducer of interferon. Proc Soc Exp Biol Med. 1970 Feb;133(2):684–687. doi: 10.3181/00379727-133-34544. [DOI] [PubMed] [Google Scholar]
  7. Chany C., Brailovsky C. Stimulating interaction between viruses (stimulons). Proc Natl Acad Sci U S A. 1967 Jan;57(1):87–94. doi: 10.1073/pnas.57.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chany C., Robbe-Maridor F. Enhancing effect of the murine sarcoma virus (MSV) on the replication of the mouse hepatitis virus (MHV) in vitro. Proc Soc Exp Biol Med. 1969 May;131(1):30–35. doi: 10.3181/00379727-131-33798. [DOI] [PubMed] [Google Scholar]
  9. Collins M. J., Jr, Parker J. C. Murine virus contaminants of leukemia viruses and transplantable tumors. J Natl Cancer Inst. 1972 Oct;49(4):1139–1143. [PubMed] [Google Scholar]
  10. FINTER N. B. QUANTITATIVE HAEMADSORPTION, A NEW ASSAY TECHNIQUE. I. ASSAY OF INTERFERON. Virology. 1964 Dec;24:589–597. doi: 10.1016/0042-6822(64)90212-0. [DOI] [PubMed] [Google Scholar]
  11. Fournier F., Rousset S., Chany C. Investigations on a tissue antagonist of interferon (TAI). Proc Soc Exp Biol Med. 1969 Dec;132(3):943–950. doi: 10.3181/00379727-132-34343. [DOI] [PubMed] [Google Scholar]
  12. GHENDON Y. Z. ON THE ABILITY OF CERTAIN VIRUSES TO BLOCK THE EFFECT OF INTERFERON. Acta Virol. 1965 Mar;9:186–187. [PubMed] [Google Scholar]
  13. Ghendon Y. Z., Balandin I. G., Babushkina L. M. On the mechanism of the ability of certain viruses to block the action of interferon. Acta Virol. 1966 May;10(3):268–270. [PubMed] [Google Scholar]
  14. Glasgow L. A. Cytomegalovirus interference in vitro. Infect Immun. 1974 Apr;9(4):702–707. doi: 10.1128/iai.9.4.702-707.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Glasgow L. A., Hanshaw J. B., Merigan T. C., Petralli J. K. Interferon and cytomegalovirus in vivo and in vitro. Proc Soc Exp Biol Med. 1967 Jul;125(3):843–849. doi: 10.3181/00379727-125-32220. [DOI] [PubMed] [Google Scholar]
  16. HENSON D., SMITH R. D. INTERFERON PRODUCTION IN VITRO BY CELLS INFECTED WITH THE MURINE SALIVARY GLAND VIRUS. Proc Soc Exp Biol Med. 1964 Nov;117:517–520. doi: 10.3181/00379727-117-29625. [DOI] [PubMed] [Google Scholar]
  17. HO M. Kinetic considerations of the inhibitory action of an interferon produced in chick cultures infected with Sindbis virus. Virology. 1962 Jun;17:262–275. doi: 10.1016/0042-6822(62)90116-2. [DOI] [PubMed] [Google Scholar]
  18. Hallum J. V., Younger J. S. Quantitative aspects of inhibition of virus replication by interferon in chick embryo cell cultures. J Bacteriol. 1966 Oct;92(4):1047–1050. doi: 10.1128/jb.92.4.1047-1050.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. ISAACS A. Action of interferon on the growth of sublethally irradiated virus. Virology. 1959 Sep;9:56–61. doi: 10.1016/0042-6822(59)90100-x. [DOI] [PubMed] [Google Scholar]
  20. LINDENMANN J., GIFFORD G. E. Studies on vaccinia virus plaque formation and its inhibition by interferon. III. A simplified plaque inhibition assay of interferon. Virology. 1963 Mar;19:302–309. doi: 10.1016/0042-6822(63)90068-0. [DOI] [PubMed] [Google Scholar]
  21. Lancz G. J., Johnson T. C. Temporal relationship between virus and interferon biosynthesis in L cells infected with Newcastle disease virus. Proc Soc Exp Biol Med. 1969 Oct;132(1):266–269. doi: 10.3181/00379727-132-34194. [DOI] [PubMed] [Google Scholar]
  22. Margolis S. A., Oie H., Levy H. B. The effect of interferon, interferon inducers or interferon induced virus resistance on subsequent interferon production. J Gen Virol. 1972 May;15(2):119–128. doi: 10.1099/0022-1317-15-2-119. [DOI] [PubMed] [Google Scholar]
  23. Oie H. K., Buckler C. E., Uhlendorf C. P., Hill D. A., Baron S. Improved assays for a variety of interferons. 1. Proc Soc Exp Biol Med. 1972 Sep;140(4):1178–1181. doi: 10.3181/00379727-140-36636. [DOI] [PubMed] [Google Scholar]
  24. Osborn J. E., Medearis D. N., Jr Studies of relationship between mouse cytomegalovirus and interferon. Proc Soc Exp Biol Med. 1966 Mar;121(3):819–824. doi: 10.3181/00379727-121-30897. [DOI] [PubMed] [Google Scholar]
  25. Rabson A. S., Tyrrell S. A., Levy H. Inhibition of human cytomegalovirus in vitro by double-stranded polyribocytidylic-inosinic acid (poly Ir.Cr). Proc Soc Exp Biol Med. 1969 Jun;131(2):495–497. doi: 10.3181/00379727-131-33909. [DOI] [PubMed] [Google Scholar]
  26. SELLERS R. F., FITZPATRICK M. An assay of interferon produced in rhesus monkey and calf kidney tissue cultures using bovine enterovirus M6 as challenge. Br J Exp Pathol. 1962 Dec;43:674–683. [PMC free article] [PubMed] [Google Scholar]
  27. Sheaff E. T., Stewart R. B. A substance enhancing virus growth and antagonistic to interferon action. Can J Microbiol. 1968 Sep;14(9):965–969. doi: 10.1139/m68-160. [DOI] [PubMed] [Google Scholar]
  28. Takemoto K. K., Baron S. Non-heritable interferon resistance in a fraction of virus populations. Proc Soc Exp Biol Med. 1966 Mar;121(3):670–675. doi: 10.3181/00379727-121-30857. [DOI] [PubMed] [Google Scholar]
  29. Thomas M. T., Gresser I. Protection par l'interféron de cellules embryonnaires humaines contre l'infection par le virus cytomégalique. C R Acad Sci Hebd Seances Acad Sci D. 1969 Jun 23;268(25):3137–3139. [PubMed] [Google Scholar]
  30. Truden J. L., Sigel M. M., Dietrich L. S. An interferon antagonist: its effect on interferon action in mengo-infected Ehrlich ascites tumor cells. Virology. 1967 Sep;33(1):95–103. doi: 10.1016/0042-6822(67)90097-9. [DOI] [PubMed] [Google Scholar]
  31. Váczi L., Horváth E., Hadházy G. Studies on the conditions of interferon production by cells infected with herpesviruses. Acta Microbiol Acad Sci Hung. 1965;12(4):345–349. [PubMed] [Google Scholar]
  32. Weissenbacher M., Galin M. A., Chowchuvech E., Schachter N., Baron S. Protection by polyinosinic-polycytidylic acid complex of rabbit eye tissue cultures infected with herpes simplex virus. Effect of neomycin and virus challenge dose. Invest Ophthalmol. 1970 Nov;9(11):857–864. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES