Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1975 Nov;12(5):1116–1120. doi: 10.1128/iai.12.5.1116-1120.1975

Retention of motility and virulence of Treponema pallidum (Nichols strain) in vitro.

S R Graves, P L Sandok, H M Jenkin, R C Johnson
PMCID: PMC415406  PMID: 1104483

Abstract

A maintenance medium for Treponema pallidum was designed to hold its Eh at the optimum for that organism, -10 to -110 mV. After 100% motile (freshly harvested) T. pallidum was inoculated into the medium, the motility of the treponemes decreased to 80% after 2 days, 50% after 3.5 days, and 0% after 9 days during incubation at 34 C. Full virulence was retained for 2 days, but it dropped rapidly thereafter, and the treponemes became avirulent by day 5.

Full text

PDF
1116

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AJELLO G., PORTNOY J., LOGAN L., OLANSKY S. Studies of the Treponema pallidum immobilization (TPI) test. IV. A simplified method of preparing the modified basal medium. Am J Syph Gonorrhea Vener Dis. 1954 Jul;38(4):288–294. [PubMed] [Google Scholar]
  2. Baseman J. B., Hayes N. S. Protein synthesis by Treponema pallidum extracted from infected rabbit tissue. Infect Immun. 1974 Dec;10(6):1350–1355. doi: 10.1128/iai.10.6.1350-1355.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DOAK G. O., FREEDMAN L. D., CLARK J. W., Jr Ionic requirements of Treponema pallidum. I. Sodium and potassium. J Bacteriol. 1959 Mar;77(3):322–327. doi: 10.1128/jb.77.3.322-327.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Metzger M., Smogór W. Study of the effect of pH and Eh values of the Nelson-Diesendruck medium on the survival of virulent Treponema pallidum. Arch Immunol Ther Exp (Warsz) 1966;14(4):445–453. [PubMed] [Google Scholar]
  5. NELSON R. A., Jr, DIESENDRUCK J. A., ZHEUTLIN H. E. C., STACK P. S., BARNETT M. Studies on treponemal immobilizing antibodies in syphilis. I. Techniques of measurement and factors influencing immobilization. J Immunol. 1951 Jun;66(6):667–685. [PubMed] [Google Scholar]
  6. Nevin T. A., Guest W. J., Geller R. C. Response of Treponema pallidum to certain nutrilites. Br J Vener Dis. 1968 Dec;44(4):274–276. doi: 10.1136/sti.44.4.274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. PORTNOY J., HARRIS A., OLANSKY S. Studies of the Treponema pallidum immobilization test. I. The effect of increased sodium thioglycollate and complement. Am J Syph Gonorrhea Vener Dis. 1953 Mar;37(2):101–105. [PubMed] [Google Scholar]
  8. RICE F. A. H., NELSON R. A., Jr The isolation from beef serum of a survival factor for Treponema pallidum. J Biol Chem. 1951 Jul;191(1):35–41. [PubMed] [Google Scholar]
  9. SAURINO V. R. A modification of the Nelson treponemal sustaining medium for use in the Treponema pallidum immobilization test. Am J Syph Gonorrhea Vener Dis. 1953 Mar;37(2):112–127. [PubMed] [Google Scholar]
  10. WEBER M. M. Factors influencing the in vitro survival of Treponema pallidum. Am J Hyg. 1960 May;71:401–417. doi: 10.1093/oxfordjournals.aje.a120123. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES