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. 1975 Dec;12(6):1405–1410. doi: 10.1128/iai.12.6.1405-1410.1975

Determination of subgroup-specific feline oncornavirus-neutralizing antibody.

J P Schaller, R G Olsen
PMCID: PMC415449  PMID: 173656

Abstract

A microneutralization assay was developed for antibody-to-subgroup-specific feline oncornaviruses. This study combines the economic advantage of a microtiter system and the quantitative focus reduction method which permits contruction of multiplicity curves for determination of virus-neutralizing titers. A twofold increase in Synder-Theilen feline sarcoma virus (ST-FeSV) on feline embryo cells decreased by approximately twofold the titer of reference goat serum prepared against Kawakami-Theilen feline leukemia virus. Similar dose effects with FeLV serotype virus preparations were not observed. An assay system utilizing FeLV serotypes on sarcoma-positive leukemia-negative cells demonstrated slightly greater sensitivity than one employing ST-FeSV on FE cells. Differential antibody responses to the three subgroup-specific feline oncornaviruses (A, B ,and C) were observed in reference goat sera. This test demonstrated good reproducibility as well as sensitivity and constitutes a significant improvement over end point dilution assay systems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A. Common genetic alterations of RNA tumour viruses grown in human cells. Nature. 1971 Apr 16;230(5294):445–447. doi: 10.1038/230445a0. [DOI] [PubMed] [Google Scholar]
  2. Bassin R. H., Tuttle N., Fischinger P. J. Rapid cell culture assay technic for murine leukaemia viruses. Nature. 1971 Feb 19;229(5286):564–566. doi: 10.1038/229564b0. [DOI] [PubMed] [Google Scholar]
  3. DULBECCO R., VOGT M., STRICKLAND A. G. A study of the basic aspects of neutralization of two animal viruses, western equine encephalitis virus and poliomyelitis virus. Virology. 1956 Apr;2(2):162–205. doi: 10.1016/0042-6822(56)90017-4. [DOI] [PubMed] [Google Scholar]
  4. Fischinger P. J., Blevins C. S., Nomura S. Simple, quantitative assay for both xenotropic murine leukemia and ecotropic feline leukemia viruses. J Virol. 1974 Jul;14(1):177–179. doi: 10.1128/jvi.14.1.177-179.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hardy W. D., Jr, Hirshaut Y., Hess P. Detection of the feline leukemia virus and other mammalian oncornaviruses by immunofluorescence. Bibl Haematol. 1973;39:778–799. doi: 10.1159/000427906. [DOI] [PubMed] [Google Scholar]
  6. Huebner R. J., Hartley J. W., Rowe W. P., Lane W. T., Capps W. I. Rescue of the defective genome of Moloney sarcoma virus from a noninfectious hamster tumor and the production of pseudotype sarcoma viruses with various murine leukemia viruses. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1164–1169. doi: 10.1073/pnas.56.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jarrett O., Laird H. M., Hay D. Restricted host range of a feline leukaemia virus. Nature. 1972 Jul 28;238(5361):220–221. doi: 10.1038/238220a0. [DOI] [PubMed] [Google Scholar]
  8. Jarrett W., Jarrett O., Mackey L., Laird H., Hardy W., Jr, Essex M. Horizontal transmission of leukemia virus and leukemia in the cat. J Natl Cancer Inst. 1973 Sep;51(3):833–841. doi: 10.1093/jnci/51.3.833. [DOI] [PubMed] [Google Scholar]
  9. Kriel R. L., Wulff H., Chin T. D. Microneutralization test for determination of rhinovirus and coxsackievirus A antibody in human diploid cells. Appl Microbiol. 1969 Apr;17(4):611–613. doi: 10.1128/am.17.4.611-613.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Olsen R. G., Yohn D. S. Demonstration of antibody in cat sera to feline oncornavirus by complement-fixation inhibition. J Natl Cancer Inst. 1972 Aug;49(2):395–403. [PubMed] [Google Scholar]
  11. Pauls F. P., Dowdle W. R. A serologic study of herpesvirus hominis strains by microneutralization tests. J Immunol. 1967 May;98(5):941–947. [PubMed] [Google Scholar]
  12. SARMA P. S., HUEBNER R. J., ARMSTRONG D. A SIMPLIFIED TISSUE CULTURE TUBE NEUTRALIZATION TEST FOR ROUS SARCOMA VIRUS ANTIBODIES. Proc Soc Exp Biol Med. 1964 Feb;115:481–486. doi: 10.3181/00379727-115-28947. [DOI] [PubMed] [Google Scholar]
  13. Sarma P. S., Log T., Huebner R. J. Trans-species rescue of defective genomes of murine sarcoma virus from hamster tumor cells with helper feline leukemia virus. Proc Natl Acad Sci U S A. 1970 Jan;65(1):81–87. doi: 10.1073/pnas.65.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sarma P. S., Log T. Subgroup classification of feline leukemia and sarcoma viruses by viral interference and neutralization tests. Virology. 1973 Jul;54(1):160–169. doi: 10.1016/0042-6822(73)90125-6. [DOI] [PubMed] [Google Scholar]
  15. Sarma P. S., Sharar A., Walters V., Gardner M. A survey of cats and humans for prevalence of feline leukemia-sarcoma virus neutralizing serum antibodies. Proc Soc Exp Biol Med. 1974 Feb;145(2):560–564. doi: 10.3181/00379727-145-37851. [DOI] [PubMed] [Google Scholar]
  16. Schäfer W., Fischinger P. J., Lange J., Pister L. Properties of mouse leukemia viruses. I. Characterization of various antisera and serological identification of viral components. Virology. 1972 Jan;47(1):197–209. doi: 10.1016/0042-6822(72)90252-8. [DOI] [PubMed] [Google Scholar]
  17. TAYLOR-ROBINSON D., TYRRELL D. A. Serological studies on some viruses isolated from common colds (rhinoviruses). Br J Exp Pathol. 1962 Jun;43:264–275. [PMC free article] [PubMed] [Google Scholar]
  18. Wheeler C. E., Jr, Briggaman R. A., Henderson R. R. Discrimination between two strains (types) of Herpes simplex virus by various modifications of the neutralization test. J Immunol. 1969 May;102(5):1179–1192. [PubMed] [Google Scholar]

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