Skip to main content
. Author manuscript; available in PMC: 2014 Sep 4.
Published in final edited form as: Science. 2011 May 13;332(6031):855–858. doi: 10.1126/science.1201618

Figure 2. Phenotypic analyses of Esp_Z modulation of Plasmodium development.

Figure 2

(A–B) Effect of Esp_Z dosage on P. falciparum oocyst formation in mosquitoes (A) and P. berghei ookinete formation in vitro (B). Circles in (A) represent the number of oocysts in an individual mosquito midgut, and the horizontal lines indicate the median number of parasites per midgut. Bars in (B) represent the mean ± the standard deviation in percentage of the number of ookinetes formed in bacteria-treated groups as compared to PBS-treated controls. (C) Temporal replication of Esp_Z in mosquito midguts following bloodmeal administration of different inoculating doses of bacteria. CFU, colony-forming unit. Bars represent the mean ± the standard deviation. (D) Effect of heat-inactivated Esp_Z bacteria on P. falciparum oocyst formation. Circles represent the same as in (A). For (A), (B) and (D), statistical significance is represented by letters above each column, with different letters signifying distinct statistical groups (p<0.05; Mann-Whitney test for (A) and (D), unpaired t-test for (B)). (E) In vivo and vitro P. falciparum development in the presence of Esp_Z. Scale bar= 10μm.