Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1976 Oct;14(4):1000–1003. doi: 10.1128/iai.14.4.1000-1003.1976

Comparison of serological tests for antibody to hepatitis A antigen, using coded specimens from individuals infected with the MS-1 strain of hepatitis A virus.

J L Dienstag, S Krugman, D C Wong, R H Purcell
PMCID: PMC415484  PMID: 186409

Abstract

To compare serological tests for antibody to hepatitis A antigen (anti-HA), we tested 15 paired serum specimens, submitted under code, from individuals infected with the MS-1 strain of hepatitis A virus. Immune electron microscopy (IEM), immune adherence hemagglutination (IAHA), and solid-phase radioimmunoassay (RIA) tests for anti-HA were performed with hepatitis A antigen (HA Ag) derived from human stool; results were also compared with previously reported titers determined by IAHA with HA Ag derived from marmoset liver. Antibody titers (IAHA and RIA) and ratings (IEM) determined with stool-derived HA Ag compared favorably, and a seroresponse to HA Ag was detected by all three methods for every serum pair tested. Differences in titers were noted between IAHA tests with liver-derived and with stool-derived HA Ag, but the discrepancies could be accounted for by differences in test technique. The agreement found in this study among the three techniques was quite good and confirms the specificity and sensitivity of tests for anti-HA that are done with stool-derived HA-Ag.

Full text

PDF
1000

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almeida J. D., Gay F. W., Wreghitt T. G. Pitfalls in the study of hepatitis A. Lancet. 1974 Sep 28;2(7883):748–751. doi: 10.1016/s0140-6736(74)90943-x. [DOI] [PubMed] [Google Scholar]
  2. Boggs J. D., Melnick J. L., Conrad M. E., Felsher B. F. Viral hepatitis. Clinical and tissue culture studies. JAMA. 1970 Nov 9;214(6):1041–1046. doi: 10.1001/jama.214.6.1041. [DOI] [PubMed] [Google Scholar]
  3. Dienstag J. L., Alling D. W., Purcell R. H. Quantitation of antibody to hepatitis A antigen by immune electron microscopy. Infect Immun. 1976 Apr;13(4):1209–1213. doi: 10.1128/iai.13.4.1209-1213.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dienstag J. L., Routenberg J. A., Purcell R. H., Hooper R. R., Harrison W. O. Foodhandler-associated outbreak of hepatitis type A. An immune electron microscopic study. Ann Intern Med. 1975 Nov;83(5):647–650. doi: 10.7326/0003-4819-83-5-647. [DOI] [PubMed] [Google Scholar]
  5. Dienstag J. L., Schulman A. N., Gerety R. J., Hoofnagle J. H., Lorenz D. E., Purcell R. H., Barker L. F. Hepatitis A antigen isolated from liver and stool: immunologic comparison of antisera prepared in guinea pigs. J Immunol. 1976 Sep;117(3):876–881. [PubMed] [Google Scholar]
  6. Feinstone S. M., Kapikian A. Z., Purceli R. H. Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness. Science. 1973 Dec 7;182(4116):1026–1028. doi: 10.1126/science.182.4116.1026. [DOI] [PubMed] [Google Scholar]
  7. Hollinger F. B., Bradley D. W., Maynard J. E., Dreesman G. R., Melnick J. L. Detection of hepatitis A viral antigen by radioimmunoassay. J Immunol. 1975 Nov;115(5):1464–1466. [PubMed] [Google Scholar]
  8. Krugman S., Friedman H., Lattimer C. Viral hepatitis, type A. Identification by specific complement fixation and immune adherence tests. N Engl J Med. 1975 May 29;292(22):1141–1143. doi: 10.1056/NEJM197505292922201. [DOI] [PubMed] [Google Scholar]
  9. Krugman S., Giles J. P., Hammond J. Infectious hepatitis. Evidence for two distinctive clinical, epidemiological, and immunological types of infection. JAMA. 1967 May 1;200(5):365–373. doi: 10.1001/jama.200.5.365. [DOI] [PubMed] [Google Scholar]
  10. Miller W. J., Provost P. J., McAleer W. J., Ittensohn O. L., Villarejos V. M., Hilleman M. R. Specific immune adherence assay for human hepatitis A antibody application to diagnostic and epidemiologic investigations. Proc Soc Exp Biol Med. 1975 May;149(1):254–261. doi: 10.3181/00379727-149-38783. [DOI] [PubMed] [Google Scholar]
  11. Moritsugu Y., Dienstag J. L., Valdesuso J., Wong D. C., Wagner J., Routenberg J. A., Purcell R. H. Purification of hepatitis A antigen from feces and detection of antigen and antibody by immune adherence hemagglutination. Infect Immun. 1976 Mar;13(3):898–908. doi: 10.1128/iai.13.3.898-908.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Provost P. J., Ittensohn O. L., Villarejos V. M., Hilleman M. R. A specific complement-fixation test for human hepatitis a employing CR326 virus antigen. Diagnosis and epidemiology. Proc Soc Exp Biol Med. 1975 Apr;148(4):962–969. doi: 10.3181/00379727-148-38669. [DOI] [PubMed] [Google Scholar]
  13. Purcell R. H., Wong D. C., Moritsugu Y., Dienstag J. L., Routenberg J. A., Boggs J. D. A microtiter solid-phase radioimmunoassay for hepatitis A antigen and antibody. J Immunol. 1976 Feb;116(2):349–356. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES