Abstract
Human peripheral blood monocytes were incubated in vitro for 6 days to allow time for transformation into macrophage-like cells. Cytophilic antibodies in typhus convalescent human serum were demonstrated by addition of Rickettsia mooseri or Rickettsia prowazeki to passively sensitized human peripheral blood monocyte-derived macrophages that were held at 4 degrees C. Rosettes of rickettsiae were found around macrophages sensitized with immune serum but not around macrophages that had been incubated with normal serum. Inhibition of rosette formation occurred if the macrophages were maintained in normal human serum before addition of immune human serum. Rosettes of R. mooseri were also formed around monocytes obtained from an individual infected with R. mooseri. If the antibody-sensitized macrophages were maintained at 34 degrees C, enhanced phagocytosis of R. mooseri or R. prowazeki occurred as compared with macrophages exposed to normal human serum before infection. However, the cytophilic antibody did not significantly inhibit the subsequent growth of R. prowazeki within the macrophages. This is in contrast to results obtained when R. prowazeki was mixed with immune serum before addition to the macrophage. In the latter case, growth of R. prowazeki was largely inhibited. The significance of antibody cytophilic for macrophages in typhus infections is discussed.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BOVARNICK M. R., MILLER J. C., SNYDER J. C. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol. 1950 Apr;59(4):509–522. doi: 10.1128/jb.59.4.509-522.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beaman L., Wisseman C. L., Jr Mechanisms of immunity in typhus infections. V. Demonstration of Rickettsia mooseri-specific antibodies in convalescent mouse and human serum cytophilic for mouse peritoneal macrophages. Infect Immun. 1976 Oct;14(4):1065–1070. doi: 10.1128/iai.14.4.1065-1070.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blaese R. M., Oppenheim J. J., Seeger R. C., Waldmann T. A. Lymphocyte-macrophage interaction in antigen induced in vitro lymphocyte transformation in patients with the Wiskott-Aldrich syndrome and other diseases with anergy. Cell Immunol. 1972 Jul;4(3):228–242. doi: 10.1016/0008-8749(72)90027-5. [DOI] [PubMed] [Google Scholar]
- Gambrill M. R., Wisseman C. L., Jr Mechanisms of immunity in typhus infections. 3. Influence of human immune serum and complement on the fate of Rickettsia mooseri within the human macrophages. Infect Immun. 1973 Oct;8(4):631–640. doi: 10.1128/iai.8.4.631-640.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gambrill M. R., Wisseman C. L., Jr Mechanisms of immunity in typhus infections. I. Multiplication of typhus rickettsiae in human macrophage cell cultures in the nonimmune system: influence of virulence of rickettsial strains and of chloramphenicol. Infect Immun. 1973 Oct;8(4):519–527. doi: 10.1128/iai.8.4.519-527.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hsu H. S., Mayo D. R. Interactions between macrophages of guinea pigs and salmonellae. 3. Bactericidal action and cytophilic antibodies of macrophages of infected guinea pigs. Infect Immun. 1973 Aug;8(2):165–172. doi: 10.1128/iai.8.2.165-172.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JENKIN C. R. The effect of opsonins on the intracellular survival of bacteria. Br J Exp Pathol. 1963 Feb;44:47–57. [PMC free article] [PubMed] [Google Scholar]
- Margolis J. M., Bigley N. J. Cytophilic macroglobulin reactive with bacterial protein in mice immunized with ribonucleic acid-protein fractions of virulent Salmonella typhimurium. Infect Immun. 1972 Sep;6(3):390–397. doi: 10.1128/iai.6.3.390-397.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silberman R., Fiset P. Method for counting Rickettsiae and Chlamydiae in purified suspensions. J Bacteriol. 1968 Jan;95(1):259–261. doi: 10.1128/jb.95.1.259-261.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wike D. A., Tallent G., Peacock M. G., Ormsbee R. A. Studies of the rickettsial plaque assay technique. Infect Immun. 1972 May;5(5):715–722. doi: 10.1128/iai.5.5.715-722.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wisseman C. L., Jr, Waddell A. D., Walsh W. T. Mechanisms of immunity in typhus infections. IV. Failure of chicken embryo cells in culture to restrict growth of antibody-sensitized Rickettsia prowazeki. Infect Immun. 1974 Mar;9(3):571–575. doi: 10.1128/iai.9.3.571-575.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]