Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1976 Nov;14(5):1113–1118. doi: 10.1128/iai.14.5.1113-1118.1976

Relationship of capsular type to biochemical and immunological properties of teichoic acid preparations from unencapsulated strains of Staphylococcus aureus.

T Ohtomo, K Yoshida, C L Clemente
PMCID: PMC415501  PMID: 977123

Abstract

We investigated the biochemical and immunological characteristics of teichoic acid preparations (TAP) obtained from four unencapsulated strains of Staphylococcus aureus which nonetheless, according to the serum-soft agar technique, produced capsular type antigen and were representative of the four types A, B, C, and D. In the agar diffusion test, TAP of each strain produced a single precipitin line only against rabbit antisera corresponding to the homologous capsular type; no lines were observed against antisera to the heterologous capsular type. All TAP were ribitol type except one, glycerol, prepared from a capsular type D strain. Major acetylglucosaminyl residues of TAP from strains having capsular type A and C antigens were attached to the polyribitol phosphate by beta-linkage, whereas TAP from a type B antigen strain had an alpha-linkage; type D antigen was attached to the polyglycerol phosphate by the beta-linkage. Chemical analyses and infrared spectrograms of these TAP further confirmed their heterogeneous nature.

Full text

PDF
1113

Images in this article

1115Image
on p.1115
1117Image
on p.1117

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BADDILEY J., BUCHANAN J. G., HARDY F. E., MARTIN R. O., RAJBHANDARY U. L., SANDERSON A. R. The structure of the ribitol teichoic acid of Staphylococcus aureus H. Biochim Biophys Acta. 1961 Sep 16;52:406–407. doi: 10.1016/0006-3002(61)90699-0. [DOI] [PubMed] [Google Scholar]
  2. DAVISON A. L., BADDILEY J., HOFSTAD T., LOSNEGARD N., OEDING P. SEROLOGICAL INVESTIGATIONS ON TEICHOIC ACIDS FROM THE WALLS OF STAPHYLOCOCCI. Nature. 1964 May 30;202:872–874. doi: 10.1038/202872a0. [DOI] [PubMed] [Google Scholar]
  3. DAVISON A. L., BADDILEY J. THE DISTRIBUTION OF TEICHOIC ACIDS IN STAPHYLOCOCCI. J Gen Microbiol. 1963 Aug;32:271–276. doi: 10.1099/00221287-32-2-271. [DOI] [PubMed] [Google Scholar]
  4. Ellwood D. C., Kelemen M. V., Baddiley J. The glycerol teichoic acid from the walls of Staphylococcus albus N.T.C.C. 7944. Biochem J. 1963 Feb;86(2):213–225. doi: 10.1042/bj0860213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Elson L. A., Morgan W. T. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27(6):1824–1828. doi: 10.1042/bj0271824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Endresen C., Grov A., Oeding P. Immunochemical studies of three cell wall polysaccharides from animal coagulase-positive staphylococci. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):382–386. doi: 10.1111/j.1699-0463.1974.tb02341.x. [DOI] [PubMed] [Google Scholar]
  7. FINKELSTEIN R. A., SULKIN S. E. Characteristics of coagulase positive and coagulase negative staphylococci in serum-soft agar. J Bacteriol. 1958 Mar;75(3):339–344. doi: 10.1128/jb.75.3.339-344.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HAUKENES G., ELLWOOD D. C., BADDILEY J., OEDING P. Serological cross-reactivity between polysaccharide A and teichoic acid of Staphylococcus aureus. Biochim Biophys Acta. 1961 Oct 28;53:425–426. doi: 10.1016/0006-3002(61)90461-9. [DOI] [PubMed] [Google Scholar]
  9. Karakawa W. W., Kane J. A. Immunochemical analysis of a galactosamine-rich teichoic acid of Staphylococcus aureus, phage type 187. J Immunol. 1971 Apr;106(4):900–906. [PubMed] [Google Scholar]
  10. Knox K. W., Wicken A. J. Serological studies on the teichoic acids of Lactobacillus plantarum. Infect Immun. 1972 Jul;6(1):43–49. doi: 10.1128/iai.6.1.43-49.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Liau D. F., Melly M. A., Hash J. H. Surface polysaccharide from Staphylococcus aureus M that contains taurine, D-aminogalacturonic acid, and D-fucosamine. J Bacteriol. 1974 Sep;119(3):913–922. doi: 10.1128/jb.119.3.913-922.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Matsuno T., Slade H. D. Composition and properties of a group A streptococcal teichoic acid. J Bacteriol. 1970 Jun;102(3):747–752. doi: 10.1128/jb.102.3.747-752.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nathenson S. G., Ishimoto N., Anderson J. S., Strominger J. L. Enzymatic synthesis and immunochemistry of alpha- and beta-N-acetylglucosaminylribitol linkages in teichoic acids from several strains of Staphylococcus aureus. J Biol Chem. 1966 Feb 10;241(3):651–658. [PubMed] [Google Scholar]
  14. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  15. Oeding P. Antigenic properties of staphylococci. Ann N Y Acad Sci. 1965 Jul 23;128(1):183–190. doi: 10.1111/j.1749-6632.1965.tb11638.x. [DOI] [PubMed] [Google Scholar]
  16. Oeding P. Wall teichoic acids in animal Staphylococcus aureus strains determined by precipitation. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Jun;81(3):327–336. doi: 10.1111/j.1699-0463.1973.tb02211.x. [DOI] [PubMed] [Google Scholar]
  17. Reeder W. J., Ekstedt R. D. Unique teichoic acid isolated from the cell walls of a strain of Staphylococcus aureus. Infect Immun. 1973 Apr;7(4):586–588. doi: 10.1128/iai.7.4.586-588.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SANDERSON A. R., STROMINGER J. L., NATHENSON S. G. Chemical structure of teichoic acid from Staphylococcus aureus, strain Copenhagen. J Biol Chem. 1962 Dec;237:3603–3613. [PubMed] [Google Scholar]
  19. Schleifer K. H., Kocur M. Classification of staphylococci based on chemical and biochemical properties. Arch Mikrobiol. 1973 Oct 4;93(1):65–85. doi: 10.1007/BF00666081. [DOI] [PubMed] [Google Scholar]
  20. Slabyj B. M., Panos C. Teichoic acid of a stabilized L-form of Streptococcus pyogenes. J Bacteriol. 1973 Jun;114(3):934–942. doi: 10.1128/jb.114.3.934-942.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. TORII M., KABAT E. A., BEZER A. E. SEPARATION OF TEICHOIC ACID OF STAPHYLOCOCCUS AUREUS INTO TWO IMMUNOLOGICALLY DISTINCT SPECIFIC POLYSACCHARIDES WITH ALPHA- AND BETA-N-ACETYLGLUCOSAMINYL LINKAGES RESPECTIVELY. ANTIGENICITY OF THEICHOIC ACIDS IN MAN. J Exp Med. 1964 Jul 1;120:13–29. doi: 10.1084/jem.120.1.13. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  23. WICKEN A. J., BADDILEY J. Structure of intracellular teichoic acids from group D streptococci. Biochem J. 1963 Apr;87:54–62. doi: 10.1042/bj0870054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yoshida K. Compact colony-forming activity and the effect of pH on compact-type growth of Staphylococcus aureus strains in serum-soft agar. Am J Clin Pathol. 1973 Mar;59(3):412–416. doi: 10.1093/ajcp/59.3.412. [DOI] [PubMed] [Google Scholar]
  25. Yoshida K. Demonstration of Serologically Different Capsular Types Among Strains of Staphylococcus aureus by the Serum-Soft Agar Technique. Infect Immun. 1971 Apr;3(4):535–539. doi: 10.1128/iai.3.4.535-539.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yoshida K. Isolation of an additional capsular-type strain of Staphylococcus aureus by the serum-soft agar technique. Infect Immun. 1972 May;5(5):833–834. doi: 10.1128/iai.5.5.833-834.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yoshida K., Nakamura A., Otomo T., Iwami S. Detection of capsular antigen production in unencapsulated strains of Staphylococcus aureus. Infect Immun. 1974 Apr;9(4):620–623. doi: 10.1128/iai.9.4.620-623.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Yoshida K., Smith M. R., Naito Y. Biological and Immunological Properties of Encapsulated Strains of Staphylococcus aureus from Human Sources. Infect Immun. 1970 Nov;2(5):528–532. doi: 10.1128/iai.2.5.528-532.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES