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. 1976 Nov;14(5):1167–1171. doi: 10.1128/iai.14.5.1167-1171.1976

The Syrian hamster: a reproducible model for studying changes in intestinal fluid secretion in response to enterotoxin challenge.

A Lepot, J G Banwell
PMCID: PMC415509  PMID: 185149

Abstract

Syrian hamsters respond in a predictable and reproducible manner to intragastric administration of purified cholera enterotoxin by intraluminal accumulation of fluid in the small bowel, cecum, and proximal colon. In the majority of animals this process is self limiting, and recovery occurs with full reabsorption of intestinal fluid by 30 to 35 h. The secretory response to 75 mug of cholera toxin has been defined, and the model was utilized to study the inhibitory effects of indomethacin, polymyxin B sulfate, glucose electrolyte solutions, and colchicine. These studies demonstrate its potential usefulness as a convenient and inexpensive technique for evaluation of pharmacological agents that might inhibit intestinal fluid secretion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banwell J. G., Sherr H. Effect of bacterial enterotoxins on the gastrointestinal tract. Gastroenterology. 1973 Sep;65(3):467–497. [PubMed] [Google Scholar]
  2. Basu S., Pickett M. J. Reaction of Vibrio cholerae and choleragenic toxin in ileal loop of laboratory animals. J Bacteriol. 1969 Nov;100(2):1142–1143. doi: 10.1128/jb.100.2.1142-1143.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blachman U., Goss S. J., Pickett M. J. Experimental cholera in the chinchilla. J Infect Dis. 1974 Apr;129(4):376–384. doi: 10.1093/infdis/129.4.376. [DOI] [PubMed] [Google Scholar]
  4. Blachman U., Graboff S. R., Haag G. E., Gottfeld E., Pickett M. J. Experimental Cholera in Chinchillas: the Immune Response in Serum and Intestinal Secretions to Vibrio cholerae and Cholera Toxin. Infect Immun. 1974 Nov;10(5):1098–1104. doi: 10.1128/iai.10.5.1098-1104.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burrows W., Musteikis G. M. Cholera infection and toxin in the rabbit ileal loop. J Infect Dis. 1966 Apr;116(2):183–190. doi: 10.1093/infdis/116.2.183. [DOI] [PubMed] [Google Scholar]
  6. Chaicumpa W., Rowley D. Experimental cholera in infant mice: protective effects of antibody. J Infect Dis. 1972 May;125(5):480–485. doi: 10.1093/infdis/125.5.480. [DOI] [PubMed] [Google Scholar]
  7. Charney A. N., Donowitz M. Prevention and reversal of cholera enterotoxin-induced intestinal secretion by methylprednisolone induction of Na+-K+-ATPase. J Clin Invest. 1976 Jun;57(6):1590–1599. doi: 10.1172/JCI108429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Farris R. K., Tapper E. J., Powell D. W., Morris S. M. Effect of aspirin on normal and cholera toxin-stimulated intestinal electrolyte transport. J Clin Invest. 1976 Apr;57(4):916–924. doi: 10.1172/JCI108368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guerrant R. L., Chen L. C., Sharp G. W. Intestinal adenyl-cyclase activity in canine cholera: correlation with fluid accumulation. J Infect Dis. 1972 Apr;125(4):377–381. doi: 10.1093/infdis/125.4.377. [DOI] [PubMed] [Google Scholar]
  10. Herbst J. J., Hurwitz R., Sunshine P., Kretchmer N. Effect of colchicine on intestinal disaccharidases: correlation with biochemical aspects of cellular renewal. J Clin Invest. 1970 Mar;49(3):530–536. doi: 10.1172/JCI106263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jacoby H. I., Marshall C. H. Antagonism of cholera enterotoxin by anti-inflammatory agents in the rat. Nature. 1972 Jan 21;235(5334):163–165. doi: 10.1038/235163a0. [DOI] [PubMed] [Google Scholar]
  12. Leitch G. J., Iwert M. E., Burrows W. Experimental cholera in the rabbit ligated ileal loop: toxin-induced water and ion movement. J Infect Dis. 1966 Jun;116(3):303–312. doi: 10.1093/infdis/116.3.303. [DOI] [PubMed] [Google Scholar]
  13. Maimon H. N., Mitch W. E., Banwell J. G., Hendrix T. R. Inhibition of enterotoxin-induced intestinal secretion by the polypeptide antibiotic, polymyxin. Johns Hopkins Med J. 1976 Mar;138(3):82–90. [PubMed] [Google Scholar]
  14. Ogawa S., Satoh K., Hamada M., Hashimoto H. In vitro culture of rabbit ova fertilized by epididymal sperms in chemically defined media. Nature. 1972 Aug 4;238(5362):270–271. doi: 10.1038/238270a0. [DOI] [PubMed] [Google Scholar]
  15. Pierce N. F., Banwell J. G., Rupak D. M., Mitra R. C., Caranasos G. J., Keimowitz R. I., Mondal A., Manji P. M. Effect of intragastric glucose-electrolyte infusion upon water and electrolyte balance in Asiatic cholera. Gastroenterology. 1968 Sep;55(3):333–343. [PubMed] [Google Scholar]
  16. Sack R. B., Carpenter C. C. Experimental canine cholera. I. Development of the model. J Infect Dis. 1969 Feb;119(2):138–149. doi: 10.1093/infdis/119.2.138. [DOI] [PubMed] [Google Scholar]
  17. Strombeck D. R. The production of intestinal fluid by cholera toxin in the rat. Proc Soc Exp Biol Med. 1972 May;140(1):297–303. doi: 10.3181/00379727-140-36444. [DOI] [PubMed] [Google Scholar]

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