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. 1976 Nov;14(5):1256–1258. doi: 10.1128/iai.14.5.1256-1258.1976

Limulus amebocyte lysate reaction with streptococcal pyrogenic exotoxin.

K W Brunson, D W Watson
PMCID: PMC415521  PMID: 789244

Abstract

Three immunologically distinct pyrogenic exotoxins derived from group A streptococci were found to cause coagulation of limulus amebocyte lysate. These exotoxins were shown to be several thousand times less active for lysate coagulation than gram-negative endotoxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BANG F. B. A bacterial disease of Limulus polyphemus. Bull Johns Hopkins Hosp. 1956 May;98(5):325–351. [PubMed] [Google Scholar]
  2. Brunson K. W., Watson D. W. Concanavalin A preparations with activities related to bacterial lipopolysaccharide. J Immunol. 1975 Aug;115(2):599–600. [PubMed] [Google Scholar]
  3. Brunson K. W., Watson D. W. Pyrogenic specificity of streptococcal exotoxins, staphylococcal enterotoxin, and gram-negative endotoxin. Infect Immun. 1974 Aug;10(2):347–351. doi: 10.1128/iai.10.2.347-351.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CREMER N., WATSON D. W. Host-parasite factors in group A streptococcal infections. A comparative study of streptococcal pyrogenic toxins and gram-negative bacterial endotoxin. J Exp Med. 1960 Dec 1;112:1037–1053. doi: 10.1084/jem.112.6.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cooper J. F., Levin J., Wagner H. N., Jr Quantitative comparison of in vitro and in vivo methods for the detection of endotoxin. J Lab Clin Med. 1971 Jul;78(1):138–148. [PubMed] [Google Scholar]
  6. Di Luzio N. R., Friedmann T. J. Letter: Bacterial endotoxins in the environment. Nature. 1973 Jul 6;244(5410):49–51. doi: 10.1038/244049a0. [DOI] [PubMed] [Google Scholar]
  7. Elin R. J., Robinson R. A., Levine A. S., Wolff S. M. Lack of clinical usefulness of the limulus test in the diagnosis of endotoxemia. N Engl J Med. 1975 Sep 11;293(11):521–524. doi: 10.1056/NEJM197509112931102. [DOI] [PubMed] [Google Scholar]
  8. Elin R. J., Wolff S. M. Nonspecificity of the limulus amebocyte lysate test: positive reactions with polynucleotides and proteins. J Infect Dis. 1973 Sep;128(3):349–352. doi: 10.1093/infdis/128.3.349. [DOI] [PubMed] [Google Scholar]
  9. Garibaldi R. A., Allman G. W., Larsen D. H., Smith C. B., Burke J. P. Detection of endotoxemia by the limulus test in patients with indwelling urinary catheters. J Infect Dis. 1973 Oct;128(4):551–554. doi: 10.1093/infdis/128.4.551. [DOI] [PubMed] [Google Scholar]
  10. Jorgensen J. H., Carvajal H. F., Chipps B. E., Smith R. F. Rapid detection of gram-negative bacteriuria by use of the Limulus endotoxin assay. Appl Microbiol. 1973 Jul;26(1):38–42. doi: 10.1128/am.26.1.38-42.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jorgensen J. H., Smith R. F. Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay. Appl Microbiol. 1973 Jul;26(1):43–48. doi: 10.1128/am.26.1.43-48.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kim Y. B., Watson D. W. A purified group A streptococcal pyrogenic exotoxin. Physiochemical and biological properties including the enhancement of susceptibility to endotoxin lethal shock. J Exp Med. 1970 Mar 1;131(3):611–622. doi: 10.1084/jem.131.3.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Krüger D., Zimmermann G., Hebold G. Ein Beitrag zur in-vitro-Pyrogenprüfung mittels des Limulus-Tests. Arzneimittelforschung. 1975 Feb;25(2):160–162. [PubMed] [Google Scholar]
  14. LEVIN J., BANG F. B. THE ROLE OF ENDOTOXIN IN THE EXTRACELLULAR COAGULATION OF LIMULUS BLOOD. Bull Johns Hopkins Hosp. 1964 Sep;115:265–274. [PubMed] [Google Scholar]
  15. Levin J., Poore T. E., Zauber N. P., Oser R. S. Detection of endotoxin in the blood of patients with sepsis due to gran-negative bacteria. N Engl J Med. 1970 Dec 10;283(24):1313–1316. doi: 10.1056/NEJM197012102832404. [DOI] [PubMed] [Google Scholar]
  16. Levin J., Tomasulo P. A., Oser R. S. Detection of endotoxin in human blood and demonstration of an inhibitor. J Lab Clin Med. 1970 Jun;75(6):903–911. [PubMed] [Google Scholar]
  17. Niwa M., Hiramatsu T., Waguri O. Proceedings: Quantitative aspects of the gelation reaction of horseshoe crab amoebocyte and bacterial endotoxin. Jpn J Med Sci Biol. 1975 Feb;28(1):98–100. [PubMed] [Google Scholar]
  18. Reinhold R. B., Fine J. A technique for quantitative measurement of endotoxin in human plasma. Proc Soc Exp Biol Med. 1971 May;137(1):334–340. doi: 10.3181/00379727-137-35572. [DOI] [PubMed] [Google Scholar]
  19. Rojas-Corona R. R., Skarnes R., Tamakuma S., Fine J. The Limulus coagulation test for endotoxin. A comparison with other assay methods. Proc Soc Exp Biol Med. 1969 Nov;132(2):599–601. doi: 10.3181/00379727-132-34268. [DOI] [PubMed] [Google Scholar]
  20. Ross S., Rodriguez W., Controni G., Korengold G., Watson S., Khan W. Limulus lysate test for gram-negative bacterial meningitis. Bedside application. JAMA. 1975 Sep 29;233(13):1366–1369. [PubMed] [Google Scholar]
  21. Rotta J., Bednár B. Biological properties of cell wall mucopeptide of hemolytic streptococci. J Exp Med. 1969 Jul 1;130(1):31–47. doi: 10.1084/jem.130.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SCHWAB J. H., WATSON D. W., CROMARTIE W. J. Production of generalized Shwartzman reaction with group A streptococcal factors. Proc Soc Exp Biol Med. 1953 Apr;82(4):754–761. doi: 10.3181/00379727-82-20237. [DOI] [PubMed] [Google Scholar]
  23. STOCK A. H., VERNEY E. Properties of scarlet fever toxin of the NY5 strain. J Immunol. 1952 Oct;69(4):373–378. [PubMed] [Google Scholar]
  24. Sullivan J. D., Jr, Watson S. W. Factors affecting the sensitivity of Limulus lysate. Appl Microbiol. 1974 Dec;28(6):1023–1026. doi: 10.1128/am.28.6.1023-1026.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WATSON D. W. Host-parasite factors in group A streptococcal infections. Pyrogenic and other effects of immunologic distinct exotoxins related to scarlet fever toxins. J Exp Med. 1960 Feb 1;111:255–284. doi: 10.1084/jem.111.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wildfeuer A., Heymer B., Schleifer K. H., Haferkamp O. Investigations on the specificity of the Limulus test for the detection of endotoxin. Appl Microbiol. 1974 Nov;28(5):867–871. doi: 10.1128/am.28.5.867-871.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wildfeuer A., Heymer B., Schleifer K. H., Seidel H. P., Haferkamp O. Zur Schockdiagnostik: Der Nachweis von Endotoxin und Mucopeptid mit dem Limulus polyphemus-Lysat-Test. Klin Wochenschr. 1974 Feb 15;52(4):175–178. doi: 10.1007/BF01614393. [DOI] [PubMed] [Google Scholar]
  28. Yin E. T. Endotoxin, thrombin, and the Limulus amebocyte lysate test. J Lab Clin Med. 1975 Sep;86(3):430–434. [PubMed] [Google Scholar]
  29. Yin E. T., Galanos C., Kinsky S., Bradshaw R. A., Wessler S., Lüderitz O., Sarmiento M. E. Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from Gram-negative bacteria. Biochim Biophys Acta. 1972 Jan 28;261(1):284–289. doi: 10.1016/0304-4165(72)90340-6. [DOI] [PubMed] [Google Scholar]
  30. Young N. S., Levin J., Prendergast R. A. An invertebrate coagulation system activated by endotoxin: evidence for enzymatic mediation. J Clin Invest. 1972 Jul;51(7):1790–1797. doi: 10.1172/JCI106980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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