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. 1976 Dec;14(6):1302–1308. doi: 10.1128/iai.14.6.1302-1308.1976

Alkaline-extracted influenza subunit vaccine.

E A Eckert
PMCID: PMC415532  PMID: 826484

Abstract

Treatment of influenza virus concentrates with alkaline solvents releases a major fraction of the viral structural protein content. As determined by polyacrylamide gel electrophoresis, the surface glycoprotein substructures, hemagglutinin and neuraminidase, are the primary solubilized products. Two forms of hemagglutinin antigen are recovered, a 39S active hemagglutinin and a 23S blocking antigen. Dose-response assays in mice demonstrate that hemagglutination-inhibiting and neuraminidase antibodies are induced. Antibody responses are comparable to those resulting from immunization with inactivated whole virus. On the basis of demonstrated purity, high yields of protective antigens, immunogenic potency, and absence of deleterious reagents, alkaline-extracted influenza protein preparations merit consideration as subunit vaccines for human use.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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