Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1976 Dec;14(6):1309–1314. doi: 10.1128/iai.14.6.1309-1314.1976

Interaction of inflammatory cells and oral microorganisms. II. Modulation of rabbit polymorphonuclear leukocyte hydrolase release by polysaccharides in response to Streptococcus mutans and Streptococcus sanguis.

W P McArthur, N S Taichman
PMCID: PMC415533  PMID: 1002299

Abstract

The release of lysosomal hydrolases from polymorphonuclear leukocytes (PMNs) has been postulated in the pathogenesis of tissue injury in periodontal disease. In the present study, lysosomal enzyme release was monitored from rabbit peritoneal exudate PMNs exposed to Streptocccus mutans or Streptococcus sanguis. S. mutans grown in brain heart infusion (BHI) broth failed to promote significant PMN enzyme release. S. sanguis grown in BHI broth, although more effective than S. mutants, was a weak stimulus for promotion of PMN hydrolase release. Preincubation of washed, viable S. mutans in sucrose or in different-molecular-weight dextrans resulted in the ability of the organisms to provoke PMN release reactions. This effect could bot be demonstrated with boiled or trypsinized S. mutans or with viable S. sanguis. However, when grown in BHI broth supplemented with sucrose, but not with glucose, both S. mutans and S. sanguis triggered discharge of PMN enzymes. The mechanism(s) whereby dextran or sucrose modulates PMN-bacterial interaction may in some manner be related to promotion of microbial adhesiveness or aggregation by dextran and by bacterial synthesis of glucans from sucrose.

Full text

PDF
1309

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Attström R. Presence of leukocytes in crevices of healthy and chronically inflamed gingivae. J Periodontal Res. 1970;5(1):42–47. doi: 10.1111/j.1600-0765.1970.tb01836.x. [DOI] [PubMed] [Google Scholar]
  2. Attström R. Studies on neutrophil polymorphonuclear leukocytes at the dento-gingival junction in gingival health and disease. J Periodontal Res Suppl. 1971;8:1–15. [PubMed] [Google Scholar]
  3. Genco R. J., Evans R. T., Ellison S. A. Dental research in microbiology with emphasis on periodontal disease. J Am Dent Assoc. 1969 May;78(5):1016–1036. doi: 10.14219/jada.archive.1969.0162. [DOI] [PubMed] [Google Scholar]
  4. Gibbons R. J., Berman K. S., Knoettner P., Kapsimalis B. Dental caries and alveolar bone loss in gnotobiotic rats infected with capsule forming streptococci of human origin. Arch Oral Biol. 1966 Jun;11(6):549–560. doi: 10.1016/0003-9969(66)90220-2. [DOI] [PubMed] [Google Scholar]
  5. Gibbons R. J., Fitzgerald R. J. Dextran-induced agglutination of Streptococcus mutans, and its potential role in the formation of microbial dental plaques. J Bacteriol. 1969 May;98(2):341–346. doi: 10.1128/jb.98.2.341-346.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gibbons R. J., Houte J. V. Bacterial adherence in oral microbial ecology. Annu Rev Microbiol. 1975;29:19–44. doi: 10.1146/annurev.mi.29.100175.000315. [DOI] [PubMed] [Google Scholar]
  7. Gibbons R. J., Nygaard M. Interbacterial aggregation of plaque bacteria. Arch Oral Biol. 1970 Dec;15(12):1397–1400. doi: 10.1016/0003-9969(70)90031-2. [DOI] [PubMed] [Google Scholar]
  8. Gibbons R. J., Nygaard M. Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Arch Oral Biol. 1968 Oct;13(10):1249–1262. doi: 10.1016/0003-9969(68)90081-2. [DOI] [PubMed] [Google Scholar]
  9. Gibbons R. J., van Houte J. On the formation of dental plaques. J Periodontol. 1973 Jun;44(6):347–360. doi: 10.1902/jop.1973.44.6.347. [DOI] [PubMed] [Google Scholar]
  10. Goldstein I. M. Polymorphonuclear Leukocyte lysosomes and immune tissue injury. Prog Allergy. 1976;20:301–340. [PubMed] [Google Scholar]
  11. Guggenheim B., Schroeder H. E. Biochemical and morphological aspects of extracellular polysaccharides produced by cariogenic streptococci. Helv Odontol Acta. 1967 Oct;11(2):131–152. [PubMed] [Google Scholar]
  12. Henson P. M. The immunologic release of constituents from neutrophil leukocytes. I. The role of antibody and complement on nonphagocytosable surfaces or phagocytosable particles. J Immunol. 1971 Dec;107(6):1535–1546. [PubMed] [Google Scholar]
  13. McArthur W. P., Baehni P., Taichman N. S. Interaction of inflammatory cells and oral microorganisms. III. Modulation of rabbit polymorphonuclear leukocyte hydrolase release response to Actinomyces viscosus and Streptococcus mutans by immunoglobulins and complement. Infect Immun. 1976 Dec;14(6):1315–1321. doi: 10.1128/iai.14.6.1315-1321.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Messner R. P., Jelinek J. Receptors for human gamma G globulin on human neutrophils. J Clin Invest. 1970 Dec;49(12):2165–2171. doi: 10.1172/JCI106435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mukasa H., Slade H. D. Mechanism of adherence of Streptococcus mutans to smooth surfaces. I. Roles of insoluble dextran-levan synthetase enzymes and cell wall polysaccharide antigen in plaque formation. Infect Immun. 1973 Oct;8(4):555–562. doi: 10.1128/iai.8.4.555-562.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schroeder H. E. Transmigration and infiltration of leucocytes in human junctional epithelium. Helv Odontol Acta. 1973 Apr;17(1):6–18. [PubMed] [Google Scholar]
  17. Socransky S. S. Relationship of bacteria to the etiology of periodontal disease. J Dent Res. 1970 Mar-Apr;49(2):203–222. doi: 10.1177/00220345700490020401. [DOI] [PubMed] [Google Scholar]
  18. Spinell D. M., Gibbons R. J. Influence of culture medium on the glucosyl transferase- and dextran-binding capacity of Streptococcus mutans 6715 cells. Infect Immun. 1974 Dec;10(6):1448–1451. doi: 10.1128/iai.10.6.1448-1451.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Taichman N. S., Freedman H. L., Uriuhara T. Inflammation and tissue injury. I. The response to intradermal injections of human dentogingival plaque in normal and leukopenic rabbits. Arch Oral Biol. 1966 Dec;11(12):1385–1392. doi: 10.1016/0003-9969(66)90028-8. [DOI] [PubMed] [Google Scholar]
  20. Taichman N. S., McArthur W. P. Interaction of inflammatory cells and oral bacteria: release of lysosomal hydrolases from rabbit polymorphonuclear leukocytes exposed to gram-positive plaque bacteria. Arch Oral Biol. 1976;21(4):257–263. doi: 10.1016/0003-9969(76)90044-3. [DOI] [PubMed] [Google Scholar]
  21. Van Houte J., Upeslacis V. N. Studies of the mechanism of sucrose-associated colonization of Streptococcus mutans on teeth of conventional rats. J Dent Res. 1976 Mar-Apr;55(2):216–222. doi: 10.1177/00220345760550020901. [DOI] [PubMed] [Google Scholar]
  22. Weissmann G., Zurier R. B., Spieler P. J., Goldstein I. M. Mechanisms of lysosomal enzyme release from leukocytes exposed to immune complexes and other particles. J Exp Med. 1971 Sep 1;134(3 Pt 2):149s–165s. [PubMed] [Google Scholar]
  23. Wright D. G., Malawista S. E. The mobilization and extracellular release of granular enzymes from human leukocytes during phagocytosis. J Cell Biol. 1972 Jun;53(3):788–797. doi: 10.1083/jcb.53.3.788. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES