Table 3.
The evaluation results of 40 cell lines for the detection of mycoplasma contamination by three different methods (microbial culture, molecular PCR and mycoalert® enzymatic assay)
| Number | NCBI code | Cell lines | Test results | ||
|---|---|---|---|---|---|
| Microbial culture | Mycoalert® | PCR | |||
| 1. | NCBI C612 | C6/36 | Negative | Negative | Negative |
| 2. | NCBI C149 | MOLT4 | Negative | Negative | Negative |
| 3. | NCBI C450 | 5,637 | Negative | Negative | Negative |
| 4. | NCBI C483 | J774A.1 | Negative | Negative | Negative |
| 5. | NCBI C135 | MCF7 | Positive | Positive | Positive |
| 6. | NCBI C516 | MOLT17 | Negative (false-negative) | Positive | Positive |
| 7. | NCBI C161 | L929 | Positive | Positive | Positive |
| 8. | NCBI C565 | QUDB | Positive | Positive | Positive |
| 9. | NCBI C540 | B16F10 | Negative (false-negative) | Positive | Positive |
| 10. | NCBI C549 | C1300 Clone NA | Positive | Positive | Positive |
| 11. | NCBI C437 | HT-1080 | Negative | Negative | Negative |
| 12. | NCBI C118 | 1321N1 | Negative | Negative | Negative |
| 13. | NCBI C131 | AGS | Positive | Positive | Positive |
| 14. | NCBI C124 | RAJI TK+ | Negative (false-negative) | Positive (weak positive) | Positive |
| 15. | NCBI C138 | RAJI TK− | Negative | Negative | Negative |
| 16. | NCBI C181 | KE-37 | Positive | Positive | Positive |
| 17. | NCBI C577 | BE(2)-C | Positive | Positive | Positive |
| 18. | NCBI C578 | MDA-MB231 | Negative (false-negative) | Positive | Positive |
| 19. | NCBI C153 | PC12 | Positive | Negative (false-negative) | Positive |
| 20. | NCBI C428 | DU145 | Positive | Positive | Positive |
| 21. | NCBI C433 | MDA-MB361 | Negative | Negative | Negative |
| 22. | NCBI C209 | SKOV3 | Negative | Negative | Negative |
| 23. | NCBI C160 | Luckes | Negative | Negative | Negative |
| 24. | NCBI C103 | BL28 | Negative | Negative | Negative |
| 25. | NCBI C146 | SW742 | Negative | Negative | Negative |
| 26. | NCBI C459 | MIA Paca-2 | Negative (false-negative) | Negative (false-negative) | Positive |
| 27. | NCBI C482 | M-NFS-60 | Negative (false-negative) | Positive | Positive |
| 28. | NCBI C456 | MEL-III | Negative (false-negative) | Positive | Positive |
| 29. | NCBI C141 | G-8 | Positive | Positive | Positive |
| 30. | NCBI C207 | SKBR3 | Positive | Positive | Positive |
| 31. | NCBI C137 | A549 | Negative | Negative | Negative |
| 32. | NCBI C143 | COS7 | Positive | Positive | Positive |
| 33. | NCBI C110 | B95.8 | Negative | Negative | Negative |
| 34. | NCBI C114 | EL4 | Positive | Positive | Positive |
| 35. | NCBI C111 | CHO | Negative | Negative | Negative |
| 36. | NCBI C515 | NB4 | Positive | Positive | Positive |
| 37. | NCBI C598 | HSKMC | Negative | Negative | Negative |
| 38. | NCBI C555 | MG63 | Positive | Positive | Positive |
| 39. | NCBI C212 | Nalm6 | Negative | Negative | Negative |
| 40. | NCBI C453 | Saos2 | Positive | Positive | Positive |