Abstract
When Brucella suis is grown in tissue cultures of normal guinea pig macrophages, the Brucella multiplies significantly without the induction of osmotically sensitive forms. In immune macrophages in the presence of normal guinea pig serum, there is a reduction in the number of intracellular Brucella and no multiplication is seen over a 72-hr period. After 6 hr of exposure to immune macrophages, however, approximately 50% of the surviving intracellular Brucella are osmotically sensitive, i.e., they will survive and grow only on medium containing 0.2 m sucrose. Brucella grown in immune macrophages, in the presence of rabbit antiserum against whole guinea pig serum, show the presence of osmotically sensitive forms, although at a reduced level compared to the number seen with immune macrophages in normal guinea pig serum.
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Selected References
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- BRAUN W., POMALES-LEBRON A., STINEBRING W. R. Interactions between mononuclear phagocytes and Brucella abortus strains of different virulence. Proc Soc Exp Biol Med. 1958 Feb;97(2):393–397. doi: 10.3181/00379727-97-23752. [DOI] [PubMed] [Google Scholar]
- FREEMAN B. A., KROSS D. J., CIRCO R. Host-parasite relationships in brucellosis. II. Destruction of macrophage cultures by Brucella of different virulence. J. J Infect Dis. 1961 May-Jun;108:333–338. doi: 10.1093/infdis/108.3.333. [DOI] [PubMed] [Google Scholar]
- FREEMAN B. A., MUSTEIKIS G. M., BURROWS W. Protoplast formation as the mechanism for immune lysis of Vibrio cholerae. Proc Soc Exp Biol Med. 1963 Jul;113:675–680. doi: 10.3181/00379727-113-28459. [DOI] [PubMed] [Google Scholar]
- FREEMAN B. A., RUMACK B. H. CYTOPATHOGENIC EFFECT OF BRUCELLA SPHEROPLASTS ON MONOCYTES IN TISSUE CULTURE. J Bacteriol. 1964 Nov;88:1310–1315. doi: 10.1128/jb.88.5.1310-1315.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FREEMAN B. A., VANA L. R. Host-parasite relationships in brucellosis. I. Infection of normal guinea pig macrophages in tissue culture. J Infect Dis. 1958 May-Jun;102(3):258–267. doi: 10.1093/infdis/102.3.258. [DOI] [PubMed] [Google Scholar]
- FREUND J. The effect of paraffin oil and mycobacteria on antibody formation and sensitization; a review. Am J Clin Pathol. 1951 Jul;21(7):645–656. doi: 10.1093/ajcp/21.7.645. [DOI] [PubMed] [Google Scholar]
- HOLLAND J. J., PICKETT M. J. A cellular basis of immunity in experimental Brucella infection. J Exp Med. 1958 Sep 1;108(3):343–360. doi: 10.1084/jem.108.3.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hatten B. A., Sulkin S. E. Intracellular Production of Brucella L Forms I. Recovery of L Forms from Tissue Culture Cells Infected with Brucella abortus. J Bacteriol. 1966 Jan;91(1):285–296. doi: 10.1128/jb.91.1.285-296.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POMALES-LEBRON A., STINEBRING W. R. Intracellular multiplication of Brucella abortus in normal and immune mononuclear phagocytes. Proc Soc Exp Biol Med. 1957 Jan;94(1):78–83. doi: 10.3181/00379727-94-22860. [DOI] [PubMed] [Google Scholar]
- RALSTON D. J., ELBERG S. S. Intramonocytic destruction of Brucella: potentiating effect of glycine on intracellular lysozyme activity. J Infect Dis. 1961 Jul-Aug;109:71–80. doi: 10.1093/infdis/109.1.71. [DOI] [PubMed] [Google Scholar]
- STINEBRING W. R. Characteristics of intracellularly grown Brucella abortus. J Infect Dis. 1962 Jul-Aug;111:17–24. doi: 10.1093/infdis/111.1.17. [DOI] [PubMed] [Google Scholar]
- STINEBRING W. R., KESSEL R. Continuous growth of Brucella abortus in mononuclear phagocytes of rats and guinea pigs. Proc Soc Exp Biol Med. 1959 Jul;101(3):412–415. doi: 10.3181/00379727-101-24962. [DOI] [PubMed] [Google Scholar]