Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1970 Feb;1(2):164–168. doi: 10.1128/iai.1.2.164-168.1970

Purification and Characterization of Two Proteins from Culture Filtrates of Mycobacterium tuberculosis H37Ra Strain

Thomas M Daniel 1, Lavenia E Ferguson 1
PMCID: PMC415872  PMID: 16557708

Abstract

Two proteins have been purified from culture filtrates of Mycobacterium tuberculosis, H37Ra strain by a procedure combining gel filtration, diethylaminoethyl (DEAE)-cellulose chromatography, and zone electrophoresis. The two proteins are similar in molecular weight but differ slightly in charge. The faster migrating protein, designated a1, is not antigenic. The slower migrating protein, designated a2, is antigenic both with respect to antisera and as a skin-testing antigen.

Full text

PDF
164

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AFFRONTI L. F., PARLETT R. C., CORNESKY R. A. ELECTROPHORESIS ON POLYACRYLAMIDE GELS OF PROTEIN AND POLYSACCHARIDE FRACTIONS FROM MYCOBACTERIUM TUBERCULOSIS. Am Rev Respir Dis. 1965 Jan;91:1–5. doi: 10.1164/arrd.1965.91.1.1. [DOI] [PubMed] [Google Scholar]
  2. Britten R. J., Roberts R. B. High-Resolution Density Gradient Sedimentation Analysis. Science. 1960 Jan 1;131(3392):32–33. doi: 10.1126/science.131.3392.32. [DOI] [PubMed] [Google Scholar]
  3. CASTELNUOVO G., DUNCAN M. E., BELLEZZA G. MYCOBACTERIAL ANTIGENS: A STUDY OF ANTIGENS IN BACTERIAL EXTRACTS AND IN CULTURE FILTRATES OF DIFFERENT AGES. Tubercle. 1964 Sep;45:246–254. doi: 10.1016/s0041-3879(64)80015-5. [DOI] [PubMed] [Google Scholar]
  4. CHAPARAS S. D., BAER H. THE IMMUNOLOGY AND CHEMISTRY OF TUBERCULIN. II. CHROMATOGRAPHY WITH SEPHADEX OF THE NONDIALYZABLE TUBERCULIN-ACTIVE CONSTITUENTS OF BCG CULTURE FILTRATE. Am Rev Respir Dis. 1964 Jan;89:41–48. doi: 10.1164/arrd.1964.89.1.41. [DOI] [PubMed] [Google Scholar]
  5. Chrambach A., Reisfeld R. A., Wyckoff M., Zaccari J. A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis. Anal Biochem. 1967 Jul;20(1):150–154. doi: 10.1016/0003-2697(67)90272-2. [DOI] [PubMed] [Google Scholar]
  6. Diena B. B., Yugi H., Wallace R., Carriere J., Greenberg L. The bentonite flocculation test in the serology of tuberculosis. I. Purification of BCG antigens. Can J Microbiol. 1968 Aug;14(8):881–885. doi: 10.1139/m68-148. [DOI] [PubMed] [Google Scholar]
  7. FUKUI Y., YONEDA M. Extracellular proteins of tubercle bacilli. III. Column chromatographical purification of the proteins of a virulent strain (H37Rv) of Mycobacterium tuberculosis. Biken J. 1961 Sep;4:187–196. [PubMed] [Google Scholar]
  8. KNIKER W. T., LABORDE J. B. THE SEPARATION OF MYCOBACTERIAL ANTIGENS BY ION-EXCHANGE CHROMATOGRAPHY. I. DETAILS OF CHROMATOGRAPHIC AND IMMUNOLOGIC PROCEDURES AND RESULTS WITH FOUR STRAINS OF M. TUBERCULOSIS. Am Rev Respir Dis. 1964 Jan;89:29–40. doi: 10.1164/arrd.1964.89.1.29. [DOI] [PubMed] [Google Scholar]
  9. LIND A. Serological studies of mycobacteria by means of diffusion-in-gel techniques. VI. Physical, chemical and immunological characteristics of precipitinogenic factors in an antigenic preparation from a culture filtrate of a virulent strain of M. tuberculosis. Int Arch Allergy Appl Immunol. 1961;19:1–45. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  12. Pickett M. J., Pedersen M. M., Goldstein D. I., Froman S. The antigens of Mycobacterium tuberculosis. Protein fractions from cytoplasm and culture filtrate. Am Rev Respir Dis. 1968 Mar;97(3):415–422. doi: 10.1164/arrd.1968.97.3.415. [DOI] [PubMed] [Google Scholar]
  13. Yoneda M., Fukui Y. Isolation, purification, and characterization of extracellular antigens of Mycobacterium tuberculosis. Am Rev Respir Dis. 1965 Dec;92(6):9–18. doi: 10.1164/arrd.1965.92.6P2.9. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES