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. 1970 Mar;1(3):300–304. doi: 10.1128/iai.1.3.300-304.1970

Conditions Affecting the Viability of Spheroplasts in Urine

Håkan Gnarpe a, Lars Edebo a,1
PMCID: PMC415896  PMID: 16557732

Abstract

Penicillin-induced spheroplasts from Escherichia coli and Proteus vulgaris, which at neutral pH had little resistance to osmotic lysis, retained viability in media of low osmolality at pH 5.0 to 5.5. In the presence of 1,000 IU of penicillin per ml (pH 5.0) in liquid media, E. coli remained in rod form without conspicuous growth. On hypotonic agar media at pH 5.2 with 1,000 IU of penicillin per ml, the bacteria grew out as L-type colonies. These findings are discussed in relation to penicillin therapy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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