Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1970 Apr;1(4):408–416. doi: 10.1128/iai.1.4.408-416.1970

Role of Glycolipids and Phosphatidylglycerol in the Serological Activity of Mycoplasma pneumoniae

S Razin a,1, B Prescott a, G Caldes a, W D James a, R M Chanock a
PMCID: PMC415913  PMID: 16557749

Abstract

Mycoplasma pneumoniae lipids were fractionated by combined silicic acid and diethylaminoethyl-cellulose chromatography. The lipid fractions were tested for complement-fixing activity and for blocking of metabolism-inhibiting antibodies in human and rabbit antisera to M. pneumoniae. Thin-layer chromatography and carbohydrate analysis of hydrolysates of the lipid fractions indicated that glycolipids were responsible for the specific serological activities of the lipid extracts of the organism. Phosphatidylglycerol, which accompanied the glycolipids, was serologically inactive when isolated free from glycolipids. Phosphatidylglycerol considerably enhanced the serological activity of the glycolipids, serving as an auxiliary lipid. Because of this auxiliary effect, minute quantities of contaminating glycolipids sufficed to render serological activity to “purified” phosphatidylgylcerol preparations, providing an explanation to the previous identification of the active lipid as a phospholipid.

Full text

PDF
408

Images in this article

411Image
on p.411
412Image
on p.412
413Image
on p.413

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alving C. R., Kinsky S. C., Haxby J. A., Kinsky C. B. Antibody binding and complement fixation by a liposomal model membrane. Biochemistry. 1969 Apr;8(4):1582–1587. doi: 10.1021/bi00832a038. [DOI] [PubMed] [Google Scholar]
  2. Beckman B. L., Kenny G. E. Immunochemical analysis of serologically active lipids of Mycoplasma pneumoniae. J Bacteriol. 1968 Oct;96(4):1171–1180. doi: 10.1128/jb.96.4.1171-1180.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  4. ENNY G. E., GRAYSTON J. T. EATON PLEUROPNEUMONIA-LIKE ORGANISM (MYCOPLASMA PNEUMONIAE) COMPLEMENT-FIXING ANTIGEN: EXTRACTION WITH ORGANIC SOLVENTS. J Immunol. 1965 Jul;95:19–25. [PubMed] [Google Scholar]
  5. Haxby J. A., Kinsky C. B., Kinsky S. C. Immune response of a liposomal model membrane. Proc Natl Acad Sci U S A. 1968 Sep;61(1):300–307. doi: 10.1073/pnas.61.1.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
  7. Lemcke R. M., Marmion B. P., Plackett P. Immunochemical analysis of Mycoplasma pneumoniae. Ann N Y Acad Sci. 1967 Jul 28;143(1):691–702. doi: 10.1111/j.1749-6632.1967.tb27715.x. [DOI] [PubMed] [Google Scholar]
  8. Marmion B. P., Plackett P., Lemcke R. M. Immunochemical analysis of Mycoplasma pneumoniae. 1. Methods of extraction and reaction of fractions from M. pneumoniae and from M. mycoides with homologous antisera and with antisera against Streptococcus MG. Aust J Exp Biol Med Sci. 1967 Apr;45(2):163–187. [PubMed] [Google Scholar]
  9. Plackett P., Marmion B. P., Shaw E. J., Lemcke R. M. Immunochemical analysis of Mycoplasma pneumoniae. 3. Separation and chemical identification of serologically active lipids. Aust J Exp Biol Med Sci. 1969 Apr;47(2):171–195. doi: 10.1038/icb.1969.19. [DOI] [PubMed] [Google Scholar]
  10. Plackett P. The synthesis of polar lipids by mycoplasma. Ann N Y Acad Sci. 1967 Jul 28;143(1):158–164. doi: 10.1111/j.1749-6632.1967.tb27655.x. [DOI] [PubMed] [Google Scholar]
  11. Prescott B., Caldes G., James W. D., Chanock R. M. Phospholipid hapten of Mycoplasma pneumoniae. Ann N Y Acad Sci. 1967 Jul 28;143(1):497–500. doi: 10.1111/j.1749-6632.1967.tb27693.x. [DOI] [PubMed] [Google Scholar]
  12. Prescott B., Sobeslavsky O., Caldes G., Chanock R. M. Isolation and characterization of fractions of Mycoplasma pneumoniae. I. Chemical and chromatographic separation. J Bacteriol. 1966 Jun;91(6):2117–2125. doi: 10.1128/jb.91.6.2117-2125.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rapport M. M., Graf L. Immunochemical reactions of lipids. Prog Allergy. 1969;13:273–331. [PubMed] [Google Scholar]
  14. Razin S. Structure and function in mycoplasma. Annu Rev Microbiol. 1969;23:317–356. doi: 10.1146/annurev.mi.23.100169.001533. [DOI] [PubMed] [Google Scholar]
  15. Shaw N. The detection of lipids on thin-layer chromatograms with the periodate-Schiff reagents. Biochim Biophys Acta. 1968 Oct 22;164(2):435–436. doi: 10.1016/0005-2760(68)90171-9. [DOI] [PubMed] [Google Scholar]
  16. Sobeslavsky O., Prescott B., James W. D., Chanock R. M. Isolation and characterization of fractions of Mycoplasma pneumoniae. II. Antigenicity and immunogenicity. J Bacteriol. 1966 Jun;91(6):2126–2138. doi: 10.1128/jb.91.6.2126-2138.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sobeslavský O., Prescott B., James W. D., Chanock R. M. Serological and immunogenic activities of different fractions of Mycoplasma pneumoniae. Ann N Y Acad Sci. 1967 Jul 28;143(1):682–690. doi: 10.1111/j.1749-6632.1967.tb27714.x. [DOI] [PubMed] [Google Scholar]
  18. Somerson N. L., James W. D., Walls B. E., Chanock R. M. Growth of Mycoplasma pneumoniae on a glass surface. Ann N Y Acad Sci. 1967 Jul 28;143(1):384–389. doi: 10.1111/j.1749-6632.1967.tb27680.x. [DOI] [PubMed] [Google Scholar]
  19. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J Hyg (Lond) 1966 Mar;64(1):91–104. doi: 10.1017/s0022172400040377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WELLS M. A., DITTMER J. C. THE USE OF SEPHADEX FOR THE REMOVAL OF NONLIPID CONTAMINANTS FROM LIPID EXTRACTS. Biochemistry. 1963 Nov-Dec;2:1259–1263. doi: 10.1021/bi00906a015. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES