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. 1970 Jul;2(1):60–64. doi: 10.1128/iai.2.1.60-64.1970

Chlorphenesin: an Antigen-Associated Immunosuppressant

H Y Whang 1, E Neter 1
PMCID: PMC415964  PMID: 16557800

Abstract

Chlorphenesin (3-p-chlorophenoxy-1,2-propanediol), when injected intravenously together with either of two common bacterial antigens, inhibits the antibody response of the rabbit. The antigens studied are those common to Enterobacteriaceae and to gram-positive bacteria. The immunosuppression is contingent upon incubation of chlorphenesin and antigen in vitro prior to administration, since separate injection of antigen and inhibitor or of mixtures without prior incubation yields undiminished antibody response. Chlorphenesin, as shown by hemagglutination-inhibition tests, does not alter the antigenic determinants, because antibody neutralization occurs in the presence or absence of the drug. The immunosuppressive effect is reversible, since precipitation of chlorphenesin at 4 C substantially restores immunogenicity. Animals immunized with antigen-drug mixtures, which fail to respond with significant antibody production, nonetheless are immunologically primed. It is concluded that chlorphenesin represents another example of antigen-associated immunosuppressants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berger F. M., Fukui G. M., Goldenbaum E. G., DeAngelo M., Chandlee G. C. The effect of chlorphenesin on the immune response. J Immunol. 1969 Apr;102(4):1024–1031. [PubMed] [Google Scholar]
  2. Berger F. M., Fukui G., Ludwig B. J., Margolin S. Selective suppression of penicillin hypersensitivity by certain phenoxy propanediols. Proc Soc Exp Biol Med. 1967 Jan;124(1):303–310. doi: 10.3181/00379727-124-31728. [DOI] [PubMed] [Google Scholar]
  3. Fukui G. M., Berger F. M., Chandlee G. C., Goldenbaum E. G. Effect of chlorphenesin on localized hemolysis in gel assay. J Bacteriol. 1968 Oct;96(4):1133–1136. doi: 10.1128/jb.96.4.1133-1136.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lichtenstein L. M., Adkinson N. F. Chlorphenesin: a new inhibitor IgE-mediated histamine release. J Immunol. 1969 Oct;103(4):866–868. [PubMed] [Google Scholar]
  5. Neter E., Whang H. Y., Lüderitz O., Westphal O. Immunological priming without production of circulating bacterial antibodies conditioned by endotoxin and its lipoid A component. Nature. 1966 Oct 22;212(5060):420–421. doi: 10.1038/212420a0. [DOI] [PubMed] [Google Scholar]
  6. RANTZ L. A., RANDALL E., ZUCKERMAN A. Hemolysis and hemagglutination by normal and immune serums of erythrocytes treated with a nonspecies specific bacterial substance. J Infect Dis. 1956 Mar-Apr;98(2):211–222. doi: 10.1093/infdis/98.2.211. [DOI] [PubMed] [Google Scholar]
  7. SUZUKI T., GORZYNSKI E. A., NETER E. SEPARATION BY ETHANOL OF COMMON AND SOMATIC ANTIGENS OF ENTEROBACTERIACEAE. J Bacteriol. 1964 Nov;88:1240–1243. doi: 10.1128/jb.88.5.1240-1243.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Whang H. Y., Chun D., Neter E. Immunosuppression by antigen-associated human serum alpha-one protein. J Immunol. 1969 Oct;103(4):824–827. [PubMed] [Google Scholar]
  9. Whang H. Y., Chun D., Yagi Y., Neter E. Immunogenicity of soluble and aggregated common enterobacterial antigen. Int Arch Allergy Appl Immunol. 1970;38(1):57–67. doi: 10.1159/000230259. [DOI] [PubMed] [Google Scholar]
  10. Whang H. Y., Lüderitz O., Westphal O., Neter E. Inhibition by lipoid A of formation of antibodies against common antigen of enterobacteriaceae. Proc Soc Exp Biol Med. 1965 Nov;120(2):371–374. doi: 10.3181/00379727-120-30540. [DOI] [PubMed] [Google Scholar]
  11. Whang H. Y., Neter E. Antigen-associated immunosuppressant: effect of serum on immune response. Science. 1969 Jan 17;163(3864):290–291. doi: 10.1126/science.163.3864.290. [DOI] [PubMed] [Google Scholar]
  12. Whang H. Y., Neter E. Further studies on effect of endotoxin on antibody response of rabbit to common antigen of Enterobacteriaceae. J Immunol. 1967 May;98(5):948–957. [PubMed] [Google Scholar]
  13. Whang H. Y., Neter E. Immunosuppression by endotoxin and its lipoid A component. Proc Soc Exp Biol Med. 1967 Mar;124(3):919–924. doi: 10.3181/00379727-124-31886. [DOI] [PubMed] [Google Scholar]
  14. Whang H. Y., Neter E. Inhibtion by cardiolipin of the antibody response to bacterial antigens. J Immunol. 1968 Mar;100(3):501–506. [PubMed] [Google Scholar]
  15. Whang H. Y., Yagi Y., Neter E. Characterization of rabbit antibodies against common bacterial antigens and their presence in the fetus. Int Arch Allergy Appl Immunol. 1967;32(4):353–365. doi: 10.1159/000229946. [DOI] [PubMed] [Google Scholar]

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