Abstract
Crude cholera toxin detoxified by treatment with Formalin showed an increased mobility and a decrease in the number of detectable protein constituents when compared with the parent toxin by disc electrophoresis. The significance of these observations in relation to the enhanced antigenic activity of cholera toxoid as compared to parent toxin is discussed.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Coleman W. H., Kaur J., Iwert M. E., Kasai G. J., Burrows W. Cholera toxins: purification and preliminary characterization of ileal loop reactive type 2 toxin. J Bacteriol. 1968 Oct;96(4):1137–1143. doi: 10.1128/jb.96.4.1137-1143.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Craig J. P. A permeability factor (toxin) found in cholera stools and culture filtrates and its neutralization by convalescent cholera sera. Nature. 1965 Aug 7;207(997):614–616. doi: 10.1038/207614a0. [DOI] [PubMed] [Google Scholar]
- Finkelstein R. A., LoSpalluto J. J. Pathogenesis of experimental cholera. Preparation and isolation of choleragen and choleragenoid. J Exp Med. 1969 Jul 1;130(1):185–202. doi: 10.1084/jem.130.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murphy S. G. Tetanus toxin and antigenic derivatives. II. Effect of protein and formaldehyde concentration on toxoid formation. J Bacteriol. 1967 Sep;94(3):586–589. doi: 10.1128/jb.94.3.586-589.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ORNSTEIN L. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY. Ann N Y Acad Sci. 1964 Dec 28;121:321–349. doi: 10.1111/j.1749-6632.1964.tb14207.x. [DOI] [PubMed] [Google Scholar]