Abstract
Amino acid, carbohydrate, and lipid components of cell walls of Brucella abortus strain 19A (low virulence) and strain 2308 (high virulence) were compared by thin layer chromatography (TLC) and by use of an amino acid analyzer. A total of 15 amino acids were detected by both chromatographic methods. Each amino acid was present in greater amounts in strain 2308 than in strain 19A when equal amounts of hydrolysates of cell wall and endotoxin-containing preparations were analyzed. A component with the same RF value as ethanolamine was present in strain 2308 cell wall hydrolysates but was not revealed by TLC of strain 19A cell wall hydrolysates. This component was not detected with the amino acid analyzer. TLC of cell walls tagged with 2,4-dinitrofluorobenzene prior to hydrolysis showed that phenylalanine was a terminal amino acid in cell walls of B. abortus strains 19A and 2308, B. suis strain 1776, and B. melitensis strain 2500. Carbohydrates detected in cell walls of strains 19A and 2308 by TLC were tentatively identified as glucose, mannose, rhamnose, and galactose. Colorimetric tests were also positive for 2-keto-3-deoxy-octulosonic acid, heptose, and dideoxyhexose. At least seven lipid components were detected by TLC of ether extracts of cell walls of strains 19A and 2308. It is suggested that one or more lipids is important in maintaining cell wall structure, because isolated cell walls rapidly became fragmented after exposure to ether.
Full text
PDF






Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anacker R. L., Bickel W. D., Haskins W. T., Milner K. C., Ribi E., Rudbach J. A. Frequency of occurrence of native hapten among enterobacterial species. J Bacteriol. 1966 Apr;91(4):1427–1433. doi: 10.1128/jb.91.4.1427-1433.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Badakhsh F. F., Foster J. W. Endotoxin-containing preparations of Brucella abortus: chemical studies. Am J Vet Res. 1970 Feb;31(2):359–363. [PubMed] [Google Scholar]
- Bobo R. A., Eagon R. G. Lipids of cell walls of Pseudomonas aeruginosa and Brucella abortus. Can J Microbiol. 1968 May;14(5):503–513. doi: 10.1139/m68-086. [DOI] [PubMed] [Google Scholar]
- Ellwood D. C., Keppie J., Smith H. The chemical basis of the virulence of Brucella abortus. 8. The identity of purified immunogenic material from culture filtrate and from the cell-wall of Brucella abortus grown in vitro. Br J Exp Pathol. 1967 Feb;48(1):28–39. [PMC free article] [PubMed] [Google Scholar]
- FOSTER J. W., COWAN R. M., MAAG T. A. Rupture of bacteria by explosive decompression. J Bacteriol. 1962 Feb;83:330–334. doi: 10.1128/jb.83.2.330-334.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FOSTER J. W., RIBI E. Immunological role of Brucella abortus cell walls. J Bacteriol. 1962 Aug;84:258–268. doi: 10.1128/jb.84.2.258-268.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huddleson I. F. IMMUNITY IN BRUCELLOSIS. Bacteriol Rev. 1942 Jun;6(2):111–142. doi: 10.1128/br.6.2.111-142.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LARSON C. L., RIBI E., MILNER K. C., LIEBERMAN J. E. A method for titrating endotoxic activity in the skin of rabbits. J Exp Med. 1960 Jan 1;111:1–20. doi: 10.1084/jem.111.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARKENSON J., SULITZEANU D., OLITZKI A. L. Immunogenic activity of Brucella cell wall. Br J Exp Pathol. 1962 Feb;43:67–76. [PMC free article] [PubMed] [Google Scholar]
- RODE L. J., OGLESBY G., SCHUHARDT V. T. The cultivation of brucellae on chemically defined media. J Bacteriol. 1950 Nov;60(5):661–668. doi: 10.1128/jb.60.5.661-668.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rimington C. Seromucoid and the bound carbohydrate of the serum proteins. Biochem J. 1940 Jun;34(6):931–940. doi: 10.1042/bj0340931. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SANGER F. The arrangement of amino acids in proteins. Adv Protein Chem. 1952;7:1–67. doi: 10.1016/s0065-3233(08)60017-0. [DOI] [PubMed] [Google Scholar]
- SLEIN M. W., SCHNELL G. W. An aldoheptose phosphate in a polysaccharide isolated from Shigella flexneri. Proc Soc Exp Biol Med. 1953 Apr;82(4):734–738. doi: 10.3181/00379727-82-20231. [DOI] [PubMed] [Google Scholar]
- Sanger F. The free amino groups of insulin. Biochem J. 1945;39(5):507–515. doi: 10.1042/bj0390507. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILLIAMS A. E., KEPPIE J., SMITH H. The chemical basis of the virulence of Brucella abortus. III. Foetal erythritol a cause of the localisation of Brucella abortus in pregnant cows. Br J Exp Pathol. 1962 Oct;43:530–537. [PMC free article] [PubMed] [Google Scholar]