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. 1970 Oct;2(4):371–375. doi: 10.1128/iai.2.4.371-375.1970

Effect of Mg2+ and Ethylenediaminetetraacetate on the In Vitro Activity of Coumermycin A1 and Novobiocin Against Gram-Negative Bacteria

R Cleeland 1, G Beskid 1, E Grunberg 1
PMCID: PMC416019  PMID: 16557848

Abstract

The effect of Mg2+ and ethylenediaminetetraacetic acid (EDTA) on the in vitro activity of coumermycin A1 and novobiocin was determined against Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis grown in Trypticase Soy Broth or defined synthetic medium. In the presence of EDTA, the activity of coumermycin A1 was significantly enhanced (8- to 40-fold) against all four organisms in the synthetic medium lacking Mg2+; novobiocin activity was increased to a lesser extent (4- to 16-fold) against three strains and remained unchanged against P. mirabilis. Addition of 200 μg of Mg2+ per ml to the synthetic medium containing EDTA completely reversed the enhancement of activity of both antibiotics by EDTA only against P. aeruginosa. The addition of Mg2+ to the synthetic medium appreciably inhibited the activity of novobiocin (sixfold) but not coumermycin A1 against E. coli, and had little or no effect on the activity of either antibiotic against the remaining organisms. K. pneumoniae showed the greatest susceptibility to both antibiotics, and addition of EDTA enhanced this activity from 4-fold to 20-fold in all of the media tested. Subinhibitory concentrations of both antibiotics induced filament formation by all four organisms in all media. In addition, lysis of K. pneumoniae cells was observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROCK T. D., BROCK M. L. Effect of novobiocin on permeability of Escherichia coli. Arch Biochem Biophys. 1959 Nov;85:176–185. doi: 10.1016/0003-9861(59)90461-8. [DOI] [PubMed] [Google Scholar]
  2. BROCK T. D. Studies on the mode of action of novobiocin. J Bacteriol. 1956 Sep;72(3):320–323. doi: 10.1128/jb.72.3.320-323.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berger J., Schocher A. J., Batcho A. D., Pecherer B., Keller O., Maricq J., Karr A. E., Vaterlaus B. P., Furlenmeier A., Speigelberg H. Production, isolation, and synthesis of the coumermycins (sugordomycins), a new streptomycete antibiotic complex. Antimicrob Agents Chemother (Bethesda) 1965;5:778–785. [PubMed] [Google Scholar]
  4. EAGLE H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med. 1955 Jul;89(3):362–364. doi: 10.3181/00379727-89-21811. [DOI] [PubMed] [Google Scholar]
  5. Fedorko J., Katz S., Allnoch H. In vitro activity of coumermycin A1. Appl Microbiol. 1969 Nov;18(5):869–873. doi: 10.1128/am.18.5.869-873.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hamilton-Miller J. M. Damaging effects of ethylenediaminetetra-acetate and penicillins on permeability barriers in Gram-negative bacteria. Biochem J. 1966 Sep;100(3):675–682. doi: 10.1042/bj1000675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kantor G. J., Deering R. A. Effect of nalidixic acid and hydroxyurea on division ability of Escherichia coli fil+ and lon- strains. J Bacteriol. 1968 Feb;95(2):520–530. doi: 10.1128/jb.95.2.520-530.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEIVE L. ACTINOMYCIN SENSITIVITY IN ESCHERICHIA COLI PRODUCED BY EDTA. Biochem Biophys Res Commun. 1965 Jan 4;18:13–17. doi: 10.1016/0006-291x(65)90874-0. [DOI] [PubMed] [Google Scholar]
  9. Muschel L. H., Gustafson L. Antibiotic, detergent, and complement sensitivity of Salmonella typhi after ethylenediaminetetraacetic acid treatment. J Bacteriol. 1968 Jun;95(6):2010–2013. doi: 10.1128/jb.95.6.2010-2013.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. REPASKE R. Lysis of gram-negative organisms and the role of versene. Biochim Biophys Acta. 1958 Nov;30(2):225–232. doi: 10.1016/0006-3002(58)90044-1. [DOI] [PubMed] [Google Scholar]
  11. Smith D. H., Davis B. D. Mode of action of novobiocin in Escherichia coli. J Bacteriol. 1967 Jan;93(1):71–79. doi: 10.1128/jb.93.1.71-79.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Swenson P. A., Setlow R. B. Effects of ultraviolet radiation on macromolecular synthesis in Escherichia coli. J Mol Biol. 1966 Jan;15(1):201–219. doi: 10.1016/s0022-2836(66)80221-8. [DOI] [PubMed] [Google Scholar]
  13. Weiser R., Asscher A. W., Wimpenny J. In vitro reversal of antibiotic resistance by ethylenediamine tetraacetic acid. Nature. 1968 Sep 28;219(5161):1365–1366. doi: 10.1038/2191365a0. [DOI] [PubMed] [Google Scholar]

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