Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1970 Oct;2(4):448–452. doi: 10.1128/iai.2.4.448-452.1970

Effect of Anti-Kupffer Cell Serum on Phagocytosis and Humoral Antibody Formation

J C Pisano 1, N R di Luzio 1
PMCID: PMC416031  PMID: 16557860

Abstract

To ascertain the effects of selective impairment of the fixed macrophage compartment on host defense, rabbit anti-rat Kupffer cell serum was raised and tested for its ability to depress phagocytosis and to serve as an immunosuppressant. The ability of the anti-Kupffer cell serum (AKS) to depress intravascular clearance rates of gelatinized RE test lipid emulsion indicated that the antiserum can functionally impair overt phagocytic activity. The phagocytic impairment was manifested by a significantly decreased liver phagocytosis of the lipid emulsion, whereas only a slight decrease in phagocytosis by the spleen was noted. When titered in vitro, AKS was cytotoxic to hepatic and splenic macrophages. Although AKS induced some degree of immunosuppressive activity, it was not as effective as antilymphocytic serum in suppressing the humoral immune response of rats to sheep red blood cells. Selectivity in the cytotoxic activity of AKS was manifested by toxicity for macrophages but not toxicity for thymocytes or splenic lymphocytes. It is suggested that AKS as a specific antimacrophage serum may be useful for assessing the contribution of the reticuloendothelial system to host defense physiology and metabolism.

Full text

PDF
448

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Argyris B. F., Plotkin D. H. Effect of antimacrophage serum on antibody production and phagocytosis in mice. J Immunol. 1969 Aug;103(2):372–373. [PubMed] [Google Scholar]
  2. Despont J. P., Cruchaud A. In vivo and in vitro effects of anti-macrophage serum. Nature. 1969 Aug 23;223(5208):838–839. doi: 10.1038/223838a0. [DOI] [PubMed] [Google Scholar]
  3. Doan C. A. The spleen: its structure and functions. Postgrad Med. 1968 May;43(5):126–131. doi: 10.1080/00325481.1968.11693251. [DOI] [PubMed] [Google Scholar]
  4. Dyminski J. W., Argyris B. F. Prolongation of allograft survival with antimacrophage serum. Transplantation. 1969 Nov;8(5):595–601. doi: 10.1097/00007890-196911000-00006. [DOI] [PubMed] [Google Scholar]
  5. FISHMAN M., ADLER F. L. Antibody formation initiated in vitro. II. Antibody synthesis in x-irradiated recipients of diffusion chambers containing nucleic acid derived from macrophages incubated with antigen. J Exp Med. 1963 Apr 1;117:595–602. doi: 10.1084/jem.117.4.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FISHMAN M. Antibody formation in vitro. J Exp Med. 1961 Dec 1;114:837–856. doi: 10.1084/jem.114.6.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FREI P. C., BENACERRAF B., THORBECKE G. J. PHAGOCYTOSIS OF THE ANTIGEN, A CRUCIAL STEP IN THE INDUCTION OF THE PRIMARY RESPONSE. Proc Natl Acad Sci U S A. 1965 Jan;53:20–23. doi: 10.1073/pnas.53.1.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grogan J. B. Alterations in phagocytic function of rats after treatment with antilymphocyte serum. J Reticuloendothel Soc. 1969 Aug;6(4):411–418. [PubMed] [Google Scholar]
  9. Hirsch M. S., Gary G. W., Jr, Murphy F. A. In vitro and in vivo properties of antimacrophage sera. J Immunol. 1969 Mar;102(3):656–661. [PubMed] [Google Scholar]
  10. Inchley C. J. The actvity of mouse Kupffer cells following intravenous injection of T4 bacteriophage. Clin Exp Immunol. 1969 Jul;5(1):173–187. [PMC free article] [PubMed] [Google Scholar]
  11. Jennings J. F., Hughes L. A. Inhibition of phagocytosis by anti-macrophage antibodies. Nature. 1969 Jan 4;221(5175):79–80. doi: 10.1038/221079a0. [DOI] [PubMed] [Google Scholar]
  12. Loewi G., Temple A., Nind A. P., Axelrad M. A study of the effects of anti-macrophage sera. Immunology. 1969 Jan;16(1):99–106. [PMC free article] [PubMed] [Google Scholar]
  13. Menkin V., Warner C. R. Studies on Inflammation: XIII. Carbohydrate Metabolism, Local Acidosis, and the Cytological Picture in Inflammation. Am J Pathol. 1937 Jan;13(1):25–44.1. [PMC free article] [PubMed] [Google Scholar]
  14. Mosier D. E. A requirement for two cell types for antibody formation in vitro. Science. 1967 Dec 22;158(3808):1573–1575. doi: 10.1126/science.158.3808.1573. [DOI] [PubMed] [Google Scholar]
  15. Panijel J., Cayeux P. Immunosuppressive effects of macrophage antiserum. Immunology. 1968 Jun;14(6):769–780. [PMC free article] [PubMed] [Google Scholar]
  16. Pisano J. C., Filkins J. P., Di Luzio N. R. Metabolic characterization of actively phagocytizing isolated rat Kupffer cells. J Reticuloendothel Soc. 1970 Jul;8(1):25–36. [PubMed] [Google Scholar]
  17. Pisano J. C., Filkins J. P., Di Luzio N. R. Phagocytic and metabolic activities of isolated rat Kupffer cells. Proc Soc Exp Biol Med. 1968 Jul;128(3):917–922. doi: 10.3181/00379727-128-33157. [DOI] [PubMed] [Google Scholar]
  18. Pisano J. C., Patterson J. T., Di Luzio N. R. Depression of phagocytic activity of the reticuloendothelial system by antilymphocytic serum. Proc Soc Exp Biol Med. 1969 Nov;132(2):517–523. doi: 10.3181/00379727-132-34250. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES