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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Biomaterials. 2012 Nov 3;34(4):912–921. doi: 10.1016/j.biomaterials.2012.10.020

Figure 2.

Figure 2

PSS-based synthetic matrices supported long-term maintenance of HUES9 in StemPro medium. (a) HUES9 cells grown on PAm6-co-PSS2 hydrogels in StemPro medium for 20 passages stained positive for NANOG (Red) and OCT4 (Green). The nuclei are stained blue with Hoescht 33342. The inset shows higher magnification images. Scale bar: 200µm (main images) and 100µm (inset images). (b) Quantitative PCR of HUES9 cells grown on PAm6-co-PSS2 hydrogels showed similar expression level of OCT4 and NANOG to that on Matrigel. (c) Representative FACS profiles of HUES9 cells grown on PAm6-co-PSS2 hydrogel and Matrigel, which again shows PAm6-co-PSS2 hydrogels can support in vitro self-renewal of HUES9 cells similar to Matrigel.