Abstract
The tumor-initiating ability of Agrobacterium tumefaciens was enhanced two- to threefold after a preinoculation exposure to the phenothiazine derivative chlorpromazine hydrochloride. The mean number of tumors initiated per viable bacterium on pinto bean leaves was greatest after a 1-hr exposure to 15 μg of the drug per ml. Chlorpromazine had no significant effect on tumor initiation when applied to previously inoculated leaves. Chlorpromazine at 10 μg/ml was bacteriostatic; concentrations of 20 μg/ml and higher were bactericidal. In contrast to mitomycin C, which also enhances A. tumefaciens infectivity, chlorpromazine failed to induce temperate phage in several lysogenic strains of A. tumefaciens. Replication of a virulent A. tumefaciens phage, however, was not inhibited by the presence of chlorpromazine.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AGARWAL R. P., GUHA A. LYTIC ACTION OF CHLORPROMAZINE HYDROCHLORIDE ON ESCHERICHIA COLI G. CELLS. Br J Pharmacol Chemother. 1965 Apr;24:466–469. doi: 10.1111/j.1476-5381.1965.tb01735.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Aaron-da Cunha M. I. Sur la libération, par les rayons x, d'un principe tumorigène contenu dans les tissus de crown gall de tabac. C R Acad Sci Hebd Seances Acad Sci D. 1969 Jan 13;268(2):318–321. [PubMed] [Google Scholar]
- BOURDON J. L. [Contribution to the study of the antibiotic properties of chlorpromazine or 4560 RP]. Ann Inst Pasteur (Paris) 1961 Dec;101:876–886. [PubMed] [Google Scholar]
- HEBERLEIN G. T., LIPPINCOTT J. A. PHOTOREVERSIBLE ULTRAVIOLET ENHANCEMENT OF INFECTIVITY IN AGROBACTERIUM TUMEFACIENS. J Bacteriol. 1965 Jun;89:1511–1514. doi: 10.1128/jb.89.6.1511-1514.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heberlein G. T., Lippincott J. A. Enhancement of Agrobacterium tumefaciens infectivity by mitomycin C. J Bacteriol. 1967 Nov;94(5):1470–1474. doi: 10.1128/jb.94.5.1470-1474.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heberlein G. T., Lippincott J. A. Ultraviolet-induced changes in the infectivity of Agrobacterium tumefaciens. J Bacteriol. 1967 Apr;93(4):1246–1253. doi: 10.1128/jb.93.4.1246-1253.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kurkdjian A., Beardsley R., Manigault P. Bactériophages d'Agrobacterium tumefaciens. II. Souche B6m lysogène dans les tissus de Pisum sativum L, et effet de la glycine sur la production des phages. Ann Inst Pasteur (Paris) 1968 Apr;114(4):555–561. [PubMed] [Google Scholar]
- Lippincott J. A., Heberlein G. T. The quantitative determination of the infectivity of Agrobacterium tumefaciens. Am J Bot. 1965 Sep;52(8):856–863. [PubMed] [Google Scholar]
- NATHAN H. A., FRIEDMAN W. Chlorpromazine affects permeability of resting cells of Tetrahymena pyriformis. Science. 1962 Mar 9;135(3506):793–794. doi: 10.1126/science.135.3506.793. [DOI] [PubMed] [Google Scholar]
- Parsons C. L., Beardsley R. E. Bacteriphage activity in homogenates of crown gall tissue. J Virol. 1968 Jun;2(6):651–651. doi: 10.1128/jvi.2.6.651-.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Quétier F., Huguet T., Guillé E. Induction of Crown-gall: partial homology between tumor-cell DNA, bacterial DNA and the G+C--rich DNA of stressed normal cells. Biochem Biophys Res Commun. 1969 Jan 6;34(1):128–133. doi: 10.1016/0006-291x(69)90538-5. [DOI] [PubMed] [Google Scholar]
- Zimmerer R. P., Hamilton R. H., Pootjes C. Isolation and morphology of temperature Agrobacterium tumefaciens bacteriophage. J Bacteriol. 1966 Sep;92(3):746–750. doi: 10.1128/jb.92.3.746-750.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]