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. 1970 Nov;2(5):679–680. doi: 10.1128/iai.2.5.679-680.1970

Amino Acid Analysis of the Isolated and Purified Components from Crystalline Toxin of Clostridium botulinum Type A

Daniel A Boroff 1,2, H Paul Meloche 1,2, Bibhuti R Dasgupta 1,2
PMCID: PMC416070  PMID: 16557897

Abstract

The toxic and hemagglutinating components of the crystalline toxin of Clostridium botulinum were analyzed for their amino acid content and were proven to be completely different proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boroff D. A., Townend R., Fleck U., DasGupta B. R. Ultracentrifugal analysis of the crystalline toxin and isolated fractions of Clostridium botulinum type A. J Biol Chem. 1966 Nov 25;241(22):5165–5167. [PubMed] [Google Scholar]
  2. DasGupta B. R., Boroff D. A. Chromatographic isolation of hemagglutinin-free neurotoxin from crystalline toxin of Clostridium botulinum type A. Biochim Biophys Acta. 1967 Dec 12;147(3):603–605. doi: 10.1016/0005-2795(67)90024-4. [DOI] [PubMed] [Google Scholar]
  3. DasGupta B. R., Boroff D. A. Separation of toxin and hemagglutinin from crystalline toxin of Clostridium botulinum type A by anion exchange chromatography and determination of their dimensions by gel filtration. J Biol Chem. 1968 Mar 10;243(5):1065–1072. [PubMed] [Google Scholar]
  4. Dasgupta B. R., Boroff D. A., Cheong K. Cation-exchange chromatography of Clostridium botulinum type A toxin on amberlite IRC-50 resin at pH 5.55. Biochim Biophys Acta. 1968 Dec 3;168(3):522–531. doi: 10.1016/0005-2795(68)90185-2. [DOI] [PubMed] [Google Scholar]
  5. Dasgupta B. R., Boroff D. A., Rothstein E. Chromatographic fractionation of the crystalline toxin of Clostridium botulinum type A. Biochem Biophys Res Commun. 1966 Mar 22;22(6):750–756. doi: 10.1016/0006-291x(66)90212-9. [DOI] [PubMed] [Google Scholar]
  6. LAMANNA C., LOWENTHAL J. P. The lack of identity between hemagglutinin and the toxin of type A botulinal organism. J Bacteriol. 1951 Jun;61(6):751–752. doi: 10.1128/jb.61.6.751-752.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Stefanye D., Schantz E. J., Spero L. Amino acid composition of crystalline botulinum toxin, type A. J Bacteriol. 1967 Jul;94(1):277–278. doi: 10.1128/jb.94.1.277-278.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. WAGMAN J. Low molecular weight forms of type A botulinum toxin. II. Action of pepsin on intact and dissociated toxin. Arch Biochem Biophys. 1963 Mar;100:414–421. doi: 10.1016/0003-9861(63)90106-1. [DOI] [PubMed] [Google Scholar]

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