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. 1970 Dec;2(6):735–741. doi: 10.1128/iai.2.6.735-741.1970

Experimentally Induced Alterations in Antigenic Properties of an Arbovirus Serotype

J R Henderson 1, Y Hoshino 2
PMCID: PMC416084  PMID: 16557909

Abstract

Antigenic properties of Getah virus were monitored during 80 serial passages in Vero tissue cultures. The parent virus lost its serotype identity by passage 80. A clone derived from the parent remained antigenically stable during passage. The experimentally induced antigenic alterations in Getah virus were attributable to the tissue culture host selection of constituent subpopulations of virus with properties unlike those of the original parent. The marked differences in neutralization titers of low- and high-passage virus were considered related to or influenced by (i) the variation in subpopulation selection of the virus during passage and (ii) the variation in availability of antigenic stimulants during preparation of antiserum.

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Selected References

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  1. ANDERSON C. R., DOWNS W. G., WATTLEY G. H., AHIN N. W., REESE A. A. Mayaro virus: a new human disease agent. II. Isolation from blood of patients in Trinidad, B.W.I. Am J Trop Med Hyg. 1957 Nov;6(6):1012–1016. doi: 10.4269/ajtmh.1957.6.1012. [DOI] [PubMed] [Google Scholar]
  2. CAUSEY O. R., CASALS J., SHOPE R. E., UDOMSAKDI S. AURA AND UNA, TWO NEW GROUP A ARTHROPOD-BORNE VIRUSES. Am J Trop Med Hyg. 1963 Sep;12:777–781. doi: 10.4269/ajtmh.1963.12.777. [DOI] [PubMed] [Google Scholar]
  3. CHOPPIN P. W., EGGERS H. J. Heterogeneity of Coxsackie B4 virus: two kinds of particles which differ in antibody sensitivity, growth rate, and plaque size. Virology. 1962 Nov;18:470–476. doi: 10.1016/0042-6822(62)90037-5. [DOI] [PubMed] [Google Scholar]
  4. CHOPPIN P. W., TAMM I. Two kinds of particles with contrasting properties in influenza A virus strains from the 1957 pandemic. Virology. 1959 Aug;8:539–542. doi: 10.1016/0042-6822(59)90059-5. [DOI] [PubMed] [Google Scholar]
  5. CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
  6. CUTCHINS E., WARREN J., JONES W. P. The antibody response to smallpox vaccination as measured by a tissue culture plaque method. J Immunol. 1960 Sep;85:275–283. [PubMed] [Google Scholar]
  7. HENDERSON J. R. IMMUNOLOGIC CHARACTERIZATION OF WESTERN EQUINE ENCEPHALOMYELITIS VIRUS STRAINS. J Immunol. 1964 Sep;93:452–461. [PubMed] [Google Scholar]
  8. HENDERSON J. R., SHAH H. H., WALLIS R. C. ANTIGENIC VARIANTS OF ARBOVIRUSES. I. THE HOST AS A DETERMINANT IN THE EVOLVEMENT OF STRAIN VARIANTS. Virology. 1965 Jun;26:326–332. doi: 10.1016/0042-6822(65)90280-1. [DOI] [PubMed] [Google Scholar]
  9. HENDERSON J. R., TAYLOR R. M. Propagation of certain arthropod-borne viruses in avian and primate cell cultures. J Immunol. 1960 Jun;84:590–598. [PubMed] [Google Scholar]
  10. Henderson J. R. Antigenic variants of arboviruses. IV. The distinctive antigenic properties of virus clones propagated in different hosts. J Immunol. 1968 Sep;101(3):451–457. [PubMed] [Google Scholar]
  11. Henderson J. R., Levine S. I., Karabatsos N., Stim T. B. Antigenic variants of arboviruses. II. Variations in antigen expressions of strains during replication in different hosts. J Immunol. 1967 Nov;99(5):925–934. [PubMed] [Google Scholar]
  12. Henderson J. R., Levine S. I., Stim T. B., Karabatsos N. Antigenic variants of arboviruses. 3. Host passage selection of constituent antigen subpopulations of strains. J Immunol. 1967 Nov;99(5):935–944. [PubMed] [Google Scholar]
  13. KARABATSOS N. HEMOLYTIC PROPERTIES OF EASTERN AND WESTERN EQUINE ENCEPHALOMYELITIS VIRUSES. J Immunol. 1963 Jul;91:76–82. [PubMed] [Google Scholar]
  14. MATSUYAMA T., OYA A., OGATA T., KOBAYASHI I., NAKAMURA T., TAKAHASHI M., KITAOKA M. Isolation of arbor viruses from mosquitoes collected at live-stock pens in Gumma Prefecture in 1959. Jpn J Med Sci Biol. 1960 Dec;13:191–198. doi: 10.7883/yoken1952.13.191. [DOI] [PubMed] [Google Scholar]
  15. Matsuyama T., Nakamura T., Isahai K., Oya A., Kobayashi M. Haruna virus, a group A arbovirus isolated from swine in Japan. Gunma J Med Sci. 1967 Jun;16(2):131–134. [PubMed] [Google Scholar]
  16. Ozaki Y., Kumagai K. Studies on the neutralization of Japanese encephalitis virus. II. Variations in reaction properties of virus to antibody during replication in PS cell cultures. J Immunol. 1969 Oct;103(4):850–856. [PubMed] [Google Scholar]
  17. ROSS R. W. The Newala epidemic. III. The virus: isolation, pathogenic properties and relationship to the epidemic. J Hyg (Lond) 1956 Jun;54(2):177–191. doi: 10.1017/s0022172400044442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Russell P. K., Nisalak A. Dengue virus identification by the plaque reduction neutralization test. J Immunol. 1967 Aug;99(2):291–296. [PubMed] [Google Scholar]
  19. SCHERER W. F., FUNKENBUSCH M., BUESCHER E. L., IZUMIT Sagiyama virus, a new group A arthropod-borne virus from Japan. I. Isolation, immunologic classification, and ecologic observations. Am J Trop Med Hyg. 1962 Mar;11:255–268. doi: 10.4269/ajtmh.1962.11.255. [DOI] [PubMed] [Google Scholar]
  20. Simizu B., Rhim J. S., Wiebenga N. H. Characterization of the Tacaribe group of arboviruses. I. Propagation and plaque assay of Tacaribe virus in a line of African green monkey kidney cells (Vero). Proc Soc Exp Biol Med. 1967 May;125(1):119–123. doi: 10.3181/00379727-125-32029. [DOI] [PubMed] [Google Scholar]
  21. WAGNER R. R., LEVEY A. H., SNYDER R. M., RATCLIFF G. A., Jr, HYATT D. F. BIOLOGIC PROPERTIES OF TWO PLAQUE VARIANTS OF VESICULAR STOMATITIS VIRUS (INDIANA SEROTYPE). J Immunol. 1963 Jul;91:112–122. [PubMed] [Google Scholar]
  22. WILLIAMS M. C., WOODALL J. P. O'nyong-nyong fever: an epidemic virus disease in East Africa. II. Isolation and some properties of the virus. Trans R Soc Trop Med Hyg. 1961 Mar;55:135–141. doi: 10.1016/0035-9203(61)90017-7. [DOI] [PubMed] [Google Scholar]
  23. Wallis C., Melnick J. L. Concentration of enteroviruses on membrane filters. J Virol. 1967 Jun;1(3):472–477. doi: 10.1128/jvi.1.3.472-477.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wallis C., Melnick J. L. Virus aggregation as the cause of the non-neutralizable persistent fraction. J Virol. 1967 Jun;1(3):478–488. doi: 10.1128/jvi.1.3.478-488.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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