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. 1971 Jan;3(1):94–99. doi: 10.1128/iai.3.1.94-99.1971

Chemotactic Activity Generated by Staphylococcal Enterotoxin B

Charles P Craig a,1, Sigurd J Normann a,2, Virginia McGann a, William S Irvin a,3
PMCID: PMC416112  PMID: 16557952

Abstract

Staphylococcal enterotoxin B (SEB) has significant toxicity for several mammalian species by both oral and parenteral routes. When highly purified SEB was incubated for 1 hr at 37 C with fresh serum from normal young adult men with little or no antibody activity for SEB, a factor(s) chemotactic for human polymorphonuclear leukocytes (PMN) was formed. Similar experiments using rabbit serum and autologous peritoneal PMN also demonstrated chemotactic activation. All chemotactic activity generated at 37 C was destroyed by heating at 56 C for 30 min. In human studies, precipitating antibody to SEB prevented generation of chemotactic activity by SEB. Based on heat lability and antibody sensitivity, the chemotactic factor(s) generated by SEB differs from that generated by endotoxin, and suggests a mechanism by which the PMN may participate in the pathophysiology of enterotoxemia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloom G. D., Haegermark O. A study on morphological changes and histamine release induced by compound 48/80 in rat peritoneal mast cells. Exp Cell Res. 1965 Dec;40(3):637–654. doi: 10.1016/0014-4827(65)90241-7. [DOI] [PubMed] [Google Scholar]
  2. CLARK W. G., BORISON H. L. PYROGENIC EFFECT OF PURIFIED STAPHYLOCOCCAL ENTEROTOXIN. J Pharmacol Exp Ther. 1963 Nov;142:237–241. [PubMed] [Google Scholar]
  3. Cline M. J., Melmon K. L., Davis W. C., Williams H. E. Mechanism of endotoxin interaction with human leucocytes. Br J Haematol. 1968 Dec;15(6):539–547. doi: 10.1111/j.1365-2141.1968.tb01576.x. [DOI] [PubMed] [Google Scholar]
  4. Crawley G. J., Black J. N., Gray I., Blanchard J. W. Clinical chemistry of staphylococcal enterotoxin poisoning in monkeys. Appl Microbiol. 1966 May;14(3):445–450. doi: 10.1128/am.14.3.445-450.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GORDON J., ROSE B., SEHON A. H. Detection of non-precipitating antibodies in sera of individuals allergic to ragweed pollen by an in vitro method. J Exp Med. 1958 Jul 1;108(1):37–51. doi: 10.1084/jem.108.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HALL H. E., ANGELOTTI R., LEWIS K. H. QUANTITATIVE DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN B IN FOOD BY GEL-DIFFUSION METHODS. Public Health Rep. 1963 Dec;78:1089–1098. [PMC free article] [PubMed] [Google Scholar]
  7. HIRSCH J. G., CHURCH A. B. Studies of phagocytosis of group A streptococci by polymorphonuclear leucocytes in vitro. J Exp Med. 1960 Mar 1;111:309–322. doi: 10.1084/jem.111.3.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hodoval L. F., Morris E. L., Crawley G. J., Beisel W. R. Pathogenesis of lethal shock after intravenous staphylococcal enterotoxin B in monkeys. Appl Microbiol. 1968 Feb;16(2):187–192. doi: 10.21236/ad0666852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keller H. U., Sorkin E. Studies on chemotaxis. I. On the chemotactic and complement-fixing activity of gamma-globulins. Immunology. 1965 Sep;9(3):241–247. [PMC free article] [PubMed] [Google Scholar]
  10. Keller H. U. Studies on chemotaxis. 3. Modification of Boyden's technique for the evaluation of chemotactic agents. Immunology. 1966 Mar;10(3):225–230. [PMC free article] [PubMed] [Google Scholar]
  11. Kent T. H. Staphylococcal enterotoxin gastroenteritis in rhesus monkeys. Am J Pathol. 1966 Mar;48(3):387–407. [PMC free article] [PubMed] [Google Scholar]
  12. Lamanna C., Carr C. J. The botulinal, tetanal, and enterostaphylococcal toxins: a review. Clin Pharmacol Ther. 1967 Mar-Apr;8(2):286–332. doi: 10.1002/cpt196782286. [DOI] [PubMed] [Google Scholar]
  13. MARTIN S. P., GREEN R. Methods for the study of surviving leukocytes: A. Preparation of cell suspension. Methods Med Res. 1958;7:136–138. [PubMed] [Google Scholar]
  14. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  15. Martin R. R., White A. The in vitro release of leukocyte histamine by staphylococcal antigens. J Immunol. 1969 Feb;102(2):437–441. [PubMed] [Google Scholar]
  16. Normann S. J., Jaeger R. F., Johnsey R. T. Pathology of experimental enterotoxemia. The in vivo localization of staphylococcal enterotoxin B. Lab Invest. 1969 Jan;20(1):17–25. [PubMed] [Google Scholar]
  17. Normann S. J., Johnsey R. T. Absence of a circulatory transport protein for staphylococcal enterotoxin B. J Bacteriol. 1968 Mar;95(3):1178–1179. doi: 10.1128/jb.95.3.1178-1179.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. OSLER A. G., STRAUSS J. H., MAYER M. M. Diagnostic complement fixation. I. A method. Am J Syph Gonorrhea Vener Dis. 1952 Mar;36(2):140–153. [PubMed] [Google Scholar]
  19. Pike R. M. Antibody heterogeneity and serological reactions. Bacteriol Rev. 1967 Jun;31(2):157–174. doi: 10.1128/br.31.2.157-174.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]
  21. Schantz E. J., Roessler W. G., Wagman J., Spero L., Dunnery D. A., Bergdoll M. S. Purification of staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1011–1016. doi: 10.1021/bi00882a005. [DOI] [PubMed] [Google Scholar]
  22. Snyderman R., Gewurz H., Mergenhagen S. E. Interactions of the complement system with endotoxic lipopolysaccharide. Generation of a factor chemotactic for polymorphonuclear leukocytes. J Exp Med. 1968 Aug 1;128(2):259–275. doi: 10.1084/jem.128.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Snyderman R., Shin H. S., Phillips J. K., Gewurz H., Mergenhagen S. E. A neutrophil chemotatic factor derived from C'5 upon interaction of guinea pig serum with endotoxin. J Immunol. 1969 Sep;103(3):413–422. [PubMed] [Google Scholar]
  24. Sugiyama H. Endotoxin-like responses induced by staphylococcal enterotoxin. J Infect Dis. 1966 Apr;116(2):162–170. doi: 10.1093/infdis/116.2.162. [DOI] [PubMed] [Google Scholar]
  25. Sugiyama H., McKissic E. M. Leukocytic response in monkeys challenged with staphylococcal enterotoxin. J Bacteriol. 1966 Aug;92(2):349–352. doi: 10.1128/jb.92.2.349-352.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. WARD P. A., COCHRANE C. G., MUELLER-EBERHARD H. J. THE ROLE OF SERUM COMPLEMENT IN CHEMOTAXIS OF LEUKOCYTES IN VITRO. J Exp Med. 1965 Aug 1;122:327–346. doi: 10.1084/jem.122.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Ward P. A. A plasmin-split fragment of C'3 as a new chemotactic factor. J Exp Med. 1967 Aug 1;126(2):189–206. doi: 10.1084/jem.126.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Ward P. A., Newman L. J. A neutrophil chemotactic factor from human C'5. J Immunol. 1969 Jan;102(1):93–99. [PubMed] [Google Scholar]

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