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. 1971 Feb;3(2):228–236. doi: 10.1128/iai.3.2.228-236.1971

Virulence and Attenuation of Murine Cytomegalovirus

June E Osborn 1, Duard L Walker 1
PMCID: PMC416136  PMID: 16557958

Abstract

Murine cytomegalovirus (MCMV) was rapidly and regularly attenuated by passage through mouse embryo cell culture. This attenuation was manifested by alteration of lethality for suckling mice and by a striking loss of capacity to multiply in liver and spleen of weanling mice. The attenuation was selective in that the passaged virus multiplied vigorously in other organs and established high titer infections in submaxillary glands and pancreas comparable to those seen with wild virus. Furthermore, attenuated virus no longer induced transient suppression of antibody and interferon responsiveness which was a regular feature of wild MCMV infection. Wild and attenuated MCMV shared the property of being poor immunogens. They induced anti-CMV complement-fixing or neutralizing antibody very slowly with barely detectable levels present at the end of the first 2 weeks of infection. The close antigenic relationship between wild and attenuated agents was demonstrated by nearly identical neutralization by a rabbit antiserum induced with wild MCMV. Furthermore, survivors of neonatal infection with attenuated virus were fully protected against subsequent challenge with potentially lethal doses of wild MCMV. Virulence could be rapidly restored by back passage in mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Churchill A. E., Chubb R. C., Baxendale W. The attenuation, with loss of oncogenicity, of the herpes-type virus of Marek's disease (strain HPRS-16) on passage in cell culture. J Gen Virol. 1969 Jun;4(4):557–564. doi: 10.1099/0022-1317-4-4-557. [DOI] [PubMed] [Google Scholar]
  2. Churchill A. E., Payne L. N., Chubb R. C. Immunization against Marek's disease using a live attenuated virus. Nature. 1969 Feb 22;221(5182):744–747. doi: 10.1038/221744a0. [DOI] [PubMed] [Google Scholar]
  3. HINZE H. C., WALKER D. L. Variation of herpes simplex virus in persistently infected tissue cultures. J Bacteriol. 1961 Oct;82:498–504. doi: 10.1128/jb.82.4.498-504.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Henson D., Neapolitan C. Pathogenesis of chronic mouse cytomegalovirus infection in submaxillary glands of C3H mice. Am J Pathol. 1970 Feb;58(2):255–267. [PMC free article] [PubMed] [Google Scholar]
  5. Lee L. F., Roizman B., Spear P. G., Kieff E. D., Burmester B. R., Nazerian K. Marek's disease herpes virus: a cytomegalovirus. Proc Natl Acad Sci U S A. 1969 Nov;64(3):952–956. doi: 10.1073/pnas.64.3.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MANNINI A., MEDEARIS D. N., Jr Mouse salivary gland virus infections. Am J Hyg. 1961 May;73:329–343. doi: 10.1093/oxfordjournals.aje.a120192. [DOI] [PubMed] [Google Scholar]
  7. Mirchamsy H., Rapp F. A new overlay for plaquing animal viruses. Proc Soc Exp Biol Med. 1968 Oct;129(1):13–17. doi: 10.3181/00379727-129-33237. [DOI] [PubMed] [Google Scholar]
  8. Osborn J. E., Blazkovec A. A., Walker D. L. Immunosuppression during acute murine cytomegalovirus infection. J Immunol. 1968 Apr;100(4):835–844. [PubMed] [Google Scholar]
  9. Osborn J. E., Medearis D. N., Jr Studies of relationship between mouse cytomegalovirus and interferon. Proc Soc Exp Biol Med. 1966 Mar;121(3):819–824. doi: 10.3181/00379727-121-30897. [DOI] [PubMed] [Google Scholar]
  10. Osborn J. E., Medearis D. N., Jr Suppression of interferon and antibody and multiplication of Newcastle disease virus in cytomegalovirus infected mice. Proc Soc Exp Biol Med. 1967 Feb;124(2):347–353. doi: 10.3181/00379727-124-31740. [DOI] [PubMed] [Google Scholar]
  11. Osborn J. E., Walker D. L. Enhancement of infectivity of murine cytomegalovirus in vitro by centrifugal inoculation. J Virol. 1968 Sep;2(9):853–858. doi: 10.1128/jvi.2.9.853-858.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ruebner B. H., Hirano T., Slusser R., Osborn J., Medearis D. N., Jr Cytomegalovirus infection. Viral ultrastructure with particular reference to the relationship of lysosomes to cytoplasmic inclusions. Am J Pathol. 1966 Jun;48(6):971–989. [PMC free article] [PubMed] [Google Scholar]
  13. Shearer G. M., Cudkowicz G., Connell M. S., Priore R. L. Cellular differentiation of the immune system of mice. I. Separate splenic antigen-sensitive units for different types of anti-sheep antibody-forming cells. J Exp Med. 1968 Sep 1;128(3):437–457. doi: 10.1084/jem.128.3.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WHEELER C. E., Jr BIOLOGIC COMPARISON OF A SYNCYTIAL AND A SMALL GIANT CELL-FORMING STRAIN OF HERPES SIMPLEX. J Immunol. 1964 Nov;93:749–756. [PubMed] [Google Scholar]

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