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. 1971 Feb;3(2):254–259. doi: 10.1128/iai.3.2.254-259.1971

Radiation, Infection, and Macrophage Function IV. Effect of Radiation on the Proliferative Abilities of Mononuclear Phagocytes in Tuberculous Lesions of Rabbits

Saroj Chandrasekhar 1,2, Kiyoshi Shima 1,2, Arthur M Dannenberg Jr 1,2, Takeshi Kambara 1,2, Jacob I Fabrikant 1,2, William G Roessler 1,2
PMCID: PMC416140  PMID: 16557962

Abstract

Rabbits were irradiated with 400 to 600 rads 2 to 3 days before they were infected with BCG or with virulent tubercle bacilli. Biopsies were periodically removed from the resulting lesions and incubated for 1 hr with 3H-thymidine in vitro under hyperbaric oxygen. Twelve to 16 days after radiation there was a reduction in the percentage of mononuclear cells (macrophages and some lymphocytes) that had incorporated 3H-thymidine in the lesions. At this time, the lesions of the irradiated group were smaller than those of controls. These results can be explained by the reduction in the number of new mononuclear cells that entered the lesions of the irradiated group and support the tenet that local mononuclear cell division occurs mainly in cells that have recently emigrated from the blood stream. An alternate, but less likely, explanation of these results would be that radiation caused the bone marrow to release a higher percentage of “defective” mononuclear cells that were unable to divide, i.e., incorporate 3H-thymidine, in the lesions. The reduction produced by radiation in both local cell infiltration and local cell division would decrease the number of macrophages available to control the tuberculous infection in the host.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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