Abstract
A weakly virulent nonencapsulated variant of Cryptococcus neoformans is described. The chemical structure and antigenicity of the soluble polysaccharides produced by the variant strain and a typical virulent strain were compared. The soluble polysaccharides produced by both strains were composed of the same constituent monosaccharides; however, the virulent strain produced a polysaccharide having a greater uronic acid content and a larger molecular size than that of the variant strain. Soluble polysaccharides from the two strains are not closely related immunologically. Soluble polysaccharide obtained from the virulent strain did not affect persistence of the variant strain in mice.
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BENHAM R. W. The genus Cryptococcus. Bacteriol Rev. 1956 Sep;20(3):189–201. doi: 10.1128/br.20.3.189-201.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BENNETT J. E., HASENCLEVER H. F. CRYPTOCOCCUS NEOFORMANS POLYSACCHARIDE: STUDIES OF SEROLOGIC PROPERTIES AND ROLE IN INFECTION. J Immunol. 1965 Jun;94:916–920. [PubMed] [Google Scholar]
- Baker P. J., Stashak P. W., Prescott B. Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells. Appl Microbiol. 1969 Mar;17(3):422–426. doi: 10.1128/am.17.3.422-426.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blandamer A., Danishefsky I. Investigations on the structure of the capsular polysaccharide from Cryptococcus neoformans type B. Biochim Biophys Acta. 1966 Apr 25;117(2):305–313. doi: 10.1016/0304-4165(66)90081-x. [DOI] [PubMed] [Google Scholar]
- Bodenhoff J. Alteration in virulence in strains of Cryptococcus neoformans resistant to amphotericin B and polymyxin B. Acta Pathol Microbiol Scand. 1969;75(1):153–168. [PubMed] [Google Scholar]
- Bulmer G. S., Sans M. D., Gunn C. M. Cryptococcus neoformans. I. Nonencapsulated mutants. J Bacteriol. 1967 Nov;94(5):1475–1479. doi: 10.1128/jb.94.5.1475-1479.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EVANS E. E., THERIAULT R. J. The antigenic compostion of Cryptococcus neoformans. IV. The use of paper chromatography for following purification of the capsular polysaccharide. J Bacteriol. 1953 May;65(5):571–577. doi: 10.1128/jb.65.5.571-577.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Farhi F., Bulmer G. S., Tacker J. R. Cryptococcus neoformans IV. The Not-So-Encapsulated Yeast. Infect Immun. 1970 Jun;1(6):526–531. doi: 10.1128/iai.1.6.526-531.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GADEBUSCH H. H., WARD P. A., FRENKEL E. P. NATURAL HOST RESISTANCE TO INFECTION WITH CRYPTOCOCCUS NEOFORMANS. III. THE EFFECT OF CRYPTOCOCCAL POLYSACCHARIDE UPON THE PHYSIOLOGY OF THE RETICULOENDOTHELIAL SYSTEM OF LABORATORY ANIMALS. J Infect Dis. 1964 Feb;114:95–106. doi: 10.1093/infdis/114.1.95. [DOI] [PubMed] [Google Scholar]
- Goren M. B., Middlebrook G. M. Protein conjugates of polysaccharide from Cryptococcus neoforman s. J Immunol. 1967 May;98(5):901–913. [PubMed] [Google Scholar]
- KASE A., METZGER J. F. Isolation of a dry variant from a mucoid strain of Cryptococcus neoformas and preliminary comparative studies. J Bacteriol. 1962 Apr;83:926–928. doi: 10.1128/jb.83.4.926-928.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KREGER-VAN RIJ N. J. Taxonomy of Cryptococcus neoformans and its variety uniguttulatus. Antonie Van Leeuwenhoek. 1961;27:59–64. doi: 10.1007/BF02538423. [DOI] [PubMed] [Google Scholar]
- STAVITSKY A. B., ARQUILLA E. R. Studies of proteins and antibodies by specific hemagglutination and hemolysis of proteinconjugated erythrocytes. Int Arch Allergy Appl Immunol. 1958;13(1-2):1–38. doi: 10.1159/000228473. [DOI] [PubMed] [Google Scholar]
- STRAUSS R. E., KLIGMAN A. M. The use of gastric mucin to lower resistance of laboratory animals to systemic fungus infections. J Infect Dis. 1951 Mar-Apr;88(2):151–155. doi: 10.1093/infdis/88.2.151. [DOI] [PubMed] [Google Scholar]
- Widra A., Van Horn G. Cryptococcosis due to an unusually slow-growing organism. Am J Med Technol. 1967 Sep-Oct;33(5):397–399. [PubMed] [Google Scholar]
- Wilson D. E., Bennett J. E., Bailey J. W. Serologic grouping of Cryptococcus neoformans. Proc Soc Exp Biol Med. 1968 Mar;127(3):820–823. doi: 10.3181/00379727-127-32812. [DOI] [PubMed] [Google Scholar]