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. 1971 Mar;3(3):429–437. doi: 10.1128/iai.3.3.429-437.1971

Tyzzer's Disease of Rabbits: Isolation and Propagation of Bacillus piliformis (Tyzzer) in Embryonated Eggs

James R Ganaway 1, Anton M Allen 1, Thomas D Moore 1
PMCID: PMC416170  PMID: 16557992

Abstract

Bacillus piliformis (Tyzzer) was isolated from the liver of rabbits with Tyzzer's disease and serially passaged in embryonated hens' eggs. Weanling rabbits given the 32nd egg passage developed lesions typical of Tyzzer's disease and died. B. piliformis was reisolated from the liver of these rabbits in embryonated eggs. Outside the host cell, the motile vegetative phase appeared to be unstable, and no means was found to preserve its viability; the results of titrations were believed to be dependent upon the resistant stage or spore. The spore withstood repeated freeze and thaw and was resistant to heat treatment of 56 C for 1 hr but not 80 C for 0.5 hr. None of several antibacterial substances tested in embryonated eggs was completely inhibitory; B. piliformis was resistant to sulfamethazine and chloramphenicol. The taxonomic position of this pleomorphic, gram-negative, sporeforming, pathogenic bacterium which appears to grow only in certain cells of several species remains unresolved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen A. M., Ganaway J. R., Moore T. D., Kinard R. F. Tyzzer's Disease Syndrome in Laboratory Rabbits. Am J Pathol. 1965 May;46(5):859–882. [PMC free article] [PubMed] [Google Scholar]
  2. COTTRAL G. E. Endogenous viruses in the egg. Ann N Y Acad Sci. 1952 Aug 8;55(2):221–235. doi: 10.1111/j.1749-6632.1952.tb26538.x. [DOI] [PubMed] [Google Scholar]
  3. Craigie J. 'Bacillus piliformis' (Tyzzer) and Tyzzer's disease of the laboratory mouse. I. Propagation of the organism in embryonated eggs. Proc R Soc Lond B Biol Sci. 1966 Jul 19;165(998):35–60. doi: 10.1098/rspb.1966.0057. [DOI] [PubMed] [Google Scholar]
  4. Craigie J. 'Bacillus piliformis' (Tyzzer) and Tyzzer's disease of the laboratory mouse. II. Mouse pathogenicity of B. piliformis growth in embryonated eggs. Proc R Soc Lond B Biol Sci. 1966 Jul 19;165(998):61–77. doi: 10.1098/rspb.1966.0058. [DOI] [PubMed] [Google Scholar]
  5. Fujiwara K. Complement fixation reaction and agar gel double diffusion test in Tyzzer's disease of mice. Jpn J Microbiol. 1967 Jun;11(2):103–117. doi: 10.1111/j.1348-0421.1967.tb00326.x. [DOI] [PubMed] [Google Scholar]
  6. Fujiwara K., Kurashina H., Matsunuma N., Takahashi R. Demonstration of peritrichous flagella of Tyzzer's disease organism. Jpn J Microbiol. 1968 Sep;12(3):361–363. doi: 10.1111/j.1348-0421.1968.tb00407.x. [DOI] [PubMed] [Google Scholar]
  7. Jonas A. M., Percy D. H., Craft J. Tyzzer's disease in the rat. Its possible relationship with megaloileitis. Arch Pathol. 1970 Dec;90(6):516–passim. [PubMed] [Google Scholar]
  8. KANAZAWA K., IMAI A. Pure culture of the pathogenic agent of Tyzzer's disease of mice. Nature. 1959 Dec 5;184(Suppl 23):1810–1811. doi: 10.1038/1841810a0. [DOI] [PubMed] [Google Scholar]
  9. Niven J. S. Tyzzer's disease in laboratory animals. Z Versuchstierkd. 1968;10(1):168–174. [PubMed] [Google Scholar]
  10. Port C. D., Richter W. R., Moise S. M. Tyzzer's disease in the gerbil (Meriones unguiculatus). Lab Anim Care. 1970 Feb;20(1):109–111. [PubMed] [Google Scholar]
  11. Rights F. L., Jackson E. B., Technician C., Smadel J. E. Observations on Tyzzer's Disease in Mice. Am J Pathol. 1947 Jul;23(4):627–635. [PMC free article] [PubMed] [Google Scholar]
  12. Stedham M. A., Bucci T. J. Spontaneous Tyzzer's disease in a rat. Lab Anim Care. 1970 Aug;20(4 Pt 1):743–746. [PubMed] [Google Scholar]
  13. TAKAGAKI Y., ITO M., FUJIWARA K., MAEJIMA M., NAIKI M., TAJIMA Y. EFFETS D'ANTIBIOTIQUES ET DE SULFAMIDES SUR LA MALADIE DE TYZZER CHEZ LA SOURIS EXP'ERIMENTALEMENT INFECT'EE. C R Seances Soc Biol Fil. 1964;158:414–418. [PubMed] [Google Scholar]
  14. White D. J., Waldron M. M. Naturally-ccurring Tyzzer's disease in the gerbil. Vet Rec. 1969 Aug 2;85(5):111–114. doi: 10.1136/vr.85.5.111. [DOI] [PubMed] [Google Scholar]

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