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. 1971 Apr;3(4):562–566. doi: 10.1128/iai.3.4.562-566.1971

Purification and Partial Characterization of a Vibrio fetus Immunogen 1

L L Myers a
PMCID: PMC416197  PMID: 16558018

Abstract

An antigen released into broth culture medium (2 mg/100 ml of culture medium) during 20-hr growth of Vibrio fetus was removed from the growth medium by salt precipitation [50 g of (NH4)2SO4/100 ml of broth] and centrifugation. About 30 mg of the postgrowth broth (PGB) antigen protein was removed from other salt precipitable material during one polyacrylamide gel electrophoretic run. The PGB antigen was further purified by using gel filtration on Sephadex G-200, and a molecular weight of 135,000 was established. The purified PGB antigen was shown to contain protein and carbohydrate but not lipid and was a glycoprotein. The antigen had an isoelectric point at pH 4.2 and an RF value of 0.30 on acrylamide gel electrophoresis. At least one PGB antigen was detected in all (31) V. fetus isolants tested. These antigens were heat labile.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem J. 1965 Sep;96(3):595–606. doi: 10.1042/bj0960595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRYNER J. H., FRANK A. H., O'BERRY P. A. Dissociation studies of Vibrios from the bovine genital tract. Am J Vet Res. 1962 Jan;23:32–41. [PubMed] [Google Scholar]
  3. Berg R. L., Jutila J. W., Firehammer B. D. A revised classification of Vibrio fetus. Am J Vet Res. 1971 Jan;32(1):11–22. [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. MORGAN W. J. Studies on the antigenic structure of Vibrio fetus. J Comp Pathol. 1959 Apr;69(2):125–140. doi: 10.1016/s0368-1742(59)80012-6. [DOI] [PubMed] [Google Scholar]
  6. Myers L. L., Berg R. L., Firehammer B. D. Immunoserologic properties of antigens from Vibrio fetus of ovine origin. Am J Vet Res. 1970 Oct;31(10):1773–1777. [PubMed] [Google Scholar]
  7. Wrigley C. W. Analytical fractionation of plant and animal proteins by gel electrofocusing. J Chromatogr. 1968 Aug 27;36(3):362–365. doi: 10.1016/s0021-9673(01)92959-0. [DOI] [PubMed] [Google Scholar]
  8. Zacharius R. M., Zell T. E., Morrison J. H., Woodlock J. J. Glycoprotein staining following electrophoresis on acrylamide gels. Anal Biochem. 1969 Jul;30(1):148–152. doi: 10.1016/0003-2697(69)90383-2. [DOI] [PubMed] [Google Scholar]

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