Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1971 May;3(5):637–641. doi: 10.1128/iai.3.5.637-641.1971

Characteristics of Cytophilic Antibody in the Mouse

George L Brown a, Philip L Carpenter a,1
PMCID: PMC416209  PMID: 16558029

Abstract

Immunoglobulin M hemagglutinin and antibody cytophilic for macrophages were produced simultaneously in mice after primary antigenic stimulation with sheep erythrocytes in saline. Antigen emulsified in Freund's complete adjuvant induced formation of cytophilic antibody (CA) in high titer. Purified cytophilic globulin was isolated by permitting it to attach to macrophages in vitro and eluting it at 56 C. The chromatographic elution pattern, molecular weight, and resistance to mercaptoethanol suggested that CA is a member of the immunoglobulin G class of immunoglobulins.

Full text

PDF
637

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYDEN S. V. CYTOPHILIC ANTIBODY IN GUINEA-PIGS WITH DELAYED-TYPE HYPERSENSITIVITY. Immunology. 1964 Jul;7:474–483. [PMC free article] [PubMed] [Google Scholar]
  2. BOYDEN S. V., SORKIN E. The adsorption of antigen by spleen cells previously treated with antiserum in vitro. Immunology. 1960 Jul;3:272–283. [PMC free article] [PubMed] [Google Scholar]
  3. Berken A., Benacerraf B. Properties of antibodies cytophilic for macrophages. J Exp Med. 1966 Jan 1;123(1):119–144. doi: 10.1084/jem.123.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berken A., Benacerraf B. Sedimentation properties of antibody cytophilic for macrophages. J Immunol. 1968 Jun;100(6):1219–1222. [PubMed] [Google Scholar]
  5. Davey M. J., Asherson G. L. Cytophilic antibody. I. Nature of the macrophage receptor. Immunology. 1967 Jan;12(1):13–20. [PMC free article] [PubMed] [Google Scholar]
  6. Ensign J. C., Wolfe R. S. Characterization of a small proteolytic enzyme which lyses bacterial cell walls. J Bacteriol. 1966 Feb;91(2):524–534. doi: 10.1128/jb.91.2.524-534.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holtzer J. D., Winkler K. C. Experimental delayed type allergy without demonstrable antibodies. I. Absence of cytophilic antibodies. Immunology. 1967 Jun;12(6):701–712. [PMC free article] [PubMed] [Google Scholar]
  8. Jonas W. E., Gurner B. W., Nelson D. S., Coombs R. R. Passive sensitization of tissue cells. 1. Passive sensitization of macrophages by guinea-pig cytophilic antibody. Int Arch Allergy Appl Immunol. 1965;28(1):87–104. [PubMed] [Google Scholar]
  9. Kay A. B., Gurner B. W., Coombs R. R. Passive sensitization of tissue cells. 3. A primate macrophage-cytophilic antibody. Int Arch Allergy Appl Immunol. 1970;37(2):113–123. doi: 10.1159/000230226. [DOI] [PubMed] [Google Scholar]
  10. Nelson D. S., Boyden S. V. Macrophage cytophilic antibodies and delayed hypersensitivity. Br Med Bull. 1967 Jan;23(1):15–20. doi: 10.1093/oxfordjournals.bmb.a070508. [DOI] [PubMed] [Google Scholar]
  11. ROWLEY D., TURNER K. J., JENKIN C. R. THE BASIS FOR IMMUNITY TO MOUSE TYPHOID. 3. CELL-BOUND ANTIBODY. Aust J Exp Biol Med Sci. 1964 Apr;42:237–248. doi: 10.1038/icb.1964.25. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES