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. 1971 May;3(5):653–658. doi: 10.1128/iai.3.5.653-658.1971

Chemical Structure and Immunological Specificity of the Streptococcal Group E Cell Wall Polysaccharide Antigen

Pandu Soprey 1, Hutton D Slade 1
PMCID: PMC416212  PMID: 16558032

Abstract

The streptococcal group E cell wall polysaccharide antigen was extracted from strain K129 cells with hot trichloroacetic acid and purified. It contained rhamnose and glucose in a 2:1 molar ratio, 2% protein, 1% phosphorus, and was free of muramic acid and glycerol. No type polysaccharide antigen was present. The reaction of specific group E rabbit antiserum with the polysaccharide was effectively inhibited by d-glucose and β-glucosides such as 1-methyl-β-d-glucose, cellobiose, and gentiobiose. The 1-methyl-α-d-glucose was one-half as effective as the beta isomer. l-Rhamnose and N-acetyl-d-glucosamine were ineffective. Partial acid hydrolysis of the antigen followed by chromatographic separation of the oligosaccharides resulted in the isolation and analysis of five fractions. These fractions were di-, tri-, and tetrasaccharides. A study of these fractions by chemical analysis, reduction with borohydride, inhibition of the antigen-antibody reaction, release of glucose by β-glucosidase, and other evidence indicate that β-d-glucose is the immunodominant sugar in the antigen. A glucose-rhamnose trisaccharide (1:2 molar ratio) was the most effective inhibitor of the precipitin reaction; the glucose was readily released by β-glucosidase, and one-half of the rhamnose was reduced with borohydride. This trisaccharide is considered to be a repeating unit in the native polysaccharide and probably has the following structure: O-β-d-glucosyl-(1-2)-O-α-l-rhamnosyl- (1-4)-l-rhamnose. A glucose-rhamnose disaccharide in which the hexose and pentose are linked as in the trisaccharide was an effective inhibitor of the precipitin reaction. Strain K129 cells do not appear to contain a type polysaccharide antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong C. H., Payne J. B. Bacteria recovered from swine affected with cervical lymphadenitis (jowl abscess). Am J Vet Res. 1969 Sep;30(9):1607–1612. [PubMed] [Google Scholar]
  2. BLEIWEIS A. S., KRAUSE R. M. THE CELL WALLS OF GROUP D STREPTOCOCCI. I. THE IMMUNOCHEMISTRY OF THE TYPE 1 CARBOHYDRATE. J Exp Med. 1965 Aug 1;122:237–249. doi: 10.1084/jem.122.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COLLIER J. R. Streptococcic lymphadenitis of the pharyngeal region of swine. J Am Vet Med Assoc. 1956 Dec 15;129(12):543–548. [PubMed] [Google Scholar]
  4. Chionglo D. T., Hayashi J. A. Structural basis of group G streptococcal antigenicity. Arch Biochem Biophys. 1969 Mar;130(1):39–47. doi: 10.1016/0003-9861(69)90006-x. [DOI] [PubMed] [Google Scholar]
  5. Colman G., Williams R. E. The cell walls of streptococci. J Gen Microbiol. 1965 Dec;41(3):375–387. doi: 10.1099/00221287-41-3-375. [DOI] [PubMed] [Google Scholar]
  6. MICHEL M. F., WILLERS J. M. IMMUNOCHEMISTRY OF GROUP F STREPTOCOCCI; ISOLATION OF GROUP SPECIFIC OLIGOSACCHARIDES. J Gen Microbiol. 1964 Dec;37:381–389. doi: 10.1099/00221287-37-3-381. [DOI] [PubMed] [Google Scholar]
  7. MOREIRA-JACOB M. The streptococci of Lancefield's group E; biochemical and serological identification of the haemolytic strains. J Gen Microbiol. 1956 Apr;14(2):268–280. doi: 10.1099/00221287-14-2-268. [DOI] [PubMed] [Google Scholar]
  8. Matsuno T., Slade H. D. Composition and properties of a group A streptococcal teichoic acid. J Bacteriol. 1970 Jun;102(3):747–752. doi: 10.1128/jb.102.3.747-752.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Payne J. B., Armstrong C. H. Somatic antigens of Streptococcus group E. II. Separation and a partial physicochemical characterization. Appl Microbiol. 1970 May;19(5):823–829. doi: 10.1128/am.19.5.823-829.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SLADE H. D., SLAMP W. C. Cell-wall composition and the grouping antigens of Streptococci. J Bacteriol. 1962 Aug;84:345–351. doi: 10.1128/jb.84.2.345-351.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Slade H. D. Extraction of Cell-Wall Polysaccharide Antigen from Streptococci. J Bacteriol. 1965 Sep;90(3):667–672. doi: 10.1128/jb.90.3.667-672.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  13. WILLERS J. M., MICHEL M. F., SYSMA M. J., WINKLER K. C. CHEMICAL ANALYSIS AND INHIBITION REACTIONS OF THE GROUP AND TYPE ANTIGENS OF GROUP F STREPTOCOCCI. J Gen Microbiol. 1964 Jul;36:95–105. doi: 10.1099/00221287-36-1-95. [DOI] [PubMed] [Google Scholar]
  14. Willers J. M., Deddish P. A., Slade H. D. Transformation of type polysaccharide antigen synthesis and hemolysin synthesis in streptococci. J Bacteriol. 1968 Oct;96(4):1225–1230. doi: 10.1128/jb.96.4.1225-1230.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. YAO J., JACOBS N. J., DEIBEL R. H., NIVEN C. F., Jr GROUP E STREPTOCOCCI. II. SEROLOGICAL CHARACTERIZATION OF STRAINS FROM SWINE CERVICAL ABSCESSES. J Infect Dis. 1964 Oct;114:333–340. doi: 10.1093/infdis/114.4.333. [DOI] [PubMed] [Google Scholar]
  16. de Moor C. E., Thal E. Beta haemolytic streptococci of the Lancefield groups E, P and U: streptococcus infrequens. Antonie Van Leeuwenhoek. 1968;34(4):377–387. doi: 10.1007/BF02046460. [DOI] [PubMed] [Google Scholar]

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