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Journal of Biomolecular Techniques : JBT logoLink to Journal of Biomolecular Techniques : JBT
. 2014 May;25(Suppl):S19.

Improved Manager of Next Generation Sequencing Orders – MANGO

J K Georgijevic 1, R Schlapbach 1, C A Fournier
PMCID: PMC4162255

Abstract

The Functional Genomics Center Zurich (FGCZ) is a joint state-of-the-art research and training facility of the ETH Zurich and the University of Zurich. With latest technologies and expert support in genomics, transcriptomics, and bioinformatics, the FGCZ carries out research projects and technology development in collaboration with the Zurich Life Science research community. The FGCZ offers services for different applications on the Illumina HiSeq2500, Illumina MiSeq, Ion Torrent, Ion Proton and PACBIO RS. At the FGCZ, we handle hundreds of NGS projects a year. We conceptualized, developed and implemented the MANGO to help manage, track, monitor and document our various and diverse NGS service orders. The MANGO works in multiple levels, first, it is a web accessible sample tracking system. It can be accessed and sample data can be added in real-time through a computer, an android tablet or an Ipad. Second, it manages multiplexing of sequencing runs because it can detect sub-optimal index combinations from various popular commericial kits and self made indices. Third, the MANGO creates well-formatted sample sheets for the various sequencers available in the FGCZ. Fourth, it can accept data in .csv format from instruments used for QC during library preparation. And lastly, it is flexible in adapting to the ever changing NGS workflows and instrumentations. In the poster, we will present the new features that have been implemented in the MANGO.


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