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. 1995 Jun 20;92(13):5973–5976. doi: 10.1073/pnas.92.13.5973

Residues in the pathway through a membrane transporter.

R T Yan 1, P C Maloney 1
PMCID: PMC41624  PMID: 7597063

Abstract

The structure of solute transporters is understood largely from analysis of their amino acid sequences, and more direct information is greatly needed. Here we report work that applies cysteine scanning mutagenesis to describe structure-function relations in UhpT, a bacterial membrane transporter. By using an impermeant SH-reactive agent to probe single-cysteine variants, we show that UhpT transmembrane segment 7 spans the membrane as an alpha-helix and that the central portion of this helix is exposed to both membrane surfaces, forming part of the translocation pathway through this transporter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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