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. 1971 Jul;4(1):6–11. doi: 10.1128/iai.4.1.6-11.1971

Some Physical Properties of K Antigens of Escherichia coli Related to Their Biological Activity

C J Howard 1, A A Glynn 1
PMCID: PMC416255  PMID: 5005287

Abstract

The ability of K antigens from Escherichia coli to inhibit the agglutination of sheep red cells by rabbit antibody has been taken as a measure of the ability of such antigens to render strains of E. coli O-inagglutinable, resistant to complement and to phagocytosis, and to increase their virulence for mice. Analysis of K antigens by chromatography on Sepharose and diethylaminoethyl cellulose columns and by immunoelectrophoresis suggested that agglutination-inhibiting activity was directly related to molecular size rather than charge. The effect of alkali treatment showed inhibitory activity to be independent of K-specific antigenic determinants and confirmed its relation to molecular weight.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolaños R., DeWitt C. W. Isolation and characterization of the K1 (L) antigen of Escherichia coli. J Bacteriol. 1966 Mar;91(3):987–996. doi: 10.1128/jb.91.3.987-996.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CEPPELLINI R., LANDY M. Suppression of blood group agglutinability of human erythrocytes by certain bacterial polysaccharides. J Exp Med. 1963 Mar 1;117:321–338. doi: 10.1084/jem.117.3.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Glynn A. A., Howard C. J. The sensitivity to complement of strains of Escherichia coli related to their K antigens. Immunology. 1970 Mar;18(3):331–346. [PMC free article] [PubMed] [Google Scholar]
  4. Howard C. J., Glynn A. A. The virulence for mice of strains of Escherichia coli related to the effects of K antigens on their resistance to phagocytosis and killing by complement. Immunology. 1971 May;20(5):767–777. [PMC free article] [PubMed] [Google Scholar]
  5. Hungerer D., Jann K., Jann B., Orskov F., Orskov I. Immunochemistry of K antigens of Escherichia coli. 4. The K antigen of E. coli O 9:K30:H12. Eur J Biochem. 1967 Jul;2(1):115–126. doi: 10.1111/j.1432-1033.1967.tb00115.x. [DOI] [PubMed] [Google Scholar]
  6. Jann K., Jann B., Schneider K. F., Orskov F., Orskov I. Immunochemistry of K antigens of Escherichia coli. 5. The K antigen of E. coli 08:K27(A):H negative. Eur J Biochem. 1968 Sep 24;5(4):456–465. doi: 10.1111/j.1432-1033.1968.tb00392.x. [DOI] [PubMed] [Google Scholar]
  7. MUSCHEL L. H. Bactericidal activity of normal serum against bacterial cultures. II. Activity against Eschericha coli strains. Proc Soc Exp Biol Med. 1960 Mar;103:632–636. doi: 10.3181/00379727-103-25619. [DOI] [PubMed] [Google Scholar]
  8. TAYLOR C. E., WALTON K. W. The molecular characteristics determining the anticomplementary activity of dextran sulphates. Br J Exp Pathol. 1957 Jun;38(3):248–255. [PMC free article] [PubMed] [Google Scholar]

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