Abstract
Vaccinia virus and African swine fever virus are deoxyribonucleic acid viruses of cytoplasmic origin. The fact that rifampin inhibits the replication of the former virus led to an investigation of its effect on African swine fever virus. The virus used was cytopathogenic to a PK-15 cell line, hemadsorbing in pig leukocyte cultures and lethal to pigs. Rifampin clearly inhibited the multiplication and cytopathogenicity of the virus in PK-15 cells. There was a 1- to 5-log reduction in virus titer depending upon the rifampin concentration, the multiplicity of infection, and the time after infection. Inhibition was greatest at a concentration of 200 μg of rifampin/ml. The drug was not viricidal per se, and the inhibition of virus replication was not due to the cell-granulating effect of rifampin since cultures which were transiently pretreated for long as 90 hr with 200 μg of drug/ml supported viral replication to the same degree as untreated cultures.
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- Becker Y., Asher Y., Himmel N., Zakay-Rones Z., Maythar B. Rifapicin inhibition of trachoma agent in vivo. Nature. 1969 Oct 4;224(5214):33–34. doi: 10.1038/224033a0. [DOI] [PubMed] [Google Scholar]
- Becker Y., Zakay-Rones Z. Rifampicin--a new antitrachoma drug. Nature. 1969 May 31;222(5196):851–853. doi: 10.1038/222851a0. [DOI] [PubMed] [Google Scholar]
- Beggs W. H., Jenne J. W. Growth Inhibition of Mycobacterium tuberculosis After Single-Pulsed Exposures to Streptomycin, Ethambutol, and Rifampin. Infect Immun. 1970 Oct;2(4):479–483. doi: 10.1128/iai.2.4.479-483.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ben-Ishai Z., Heller E., Goldblum N., Becker Y. Rifampicin and poxvirus replication. Nature. 1969 Oct 4;224(5214):29–32. doi: 10.1038/224029a0. [DOI] [PubMed] [Google Scholar]
- Breese S. S., Jr, DeBoer C. J. Chemical structure of African swine fever virus investigated by electron microscopy. J Gen Virol. 1967 Apr;1(2):251–252. doi: 10.1099/0022-1317-1-2-251. [DOI] [PubMed] [Google Scholar]
- Geiduschek E. P., Sklar J. Continual requirement for a host RNA polymerase component in a bacteriophage development. Nature. 1969 Mar 1;221(5183):833–836. doi: 10.1038/221833a0. [DOI] [PubMed] [Google Scholar]
- Heller E., Argaman M., Levy H., Goldblum N. Selective inhibition of vaccinia virus by the antibiotic rifampicin. Nature. 1969 Apr 19;222(5190):273–274. doi: 10.1038/222273a0. [DOI] [PubMed] [Google Scholar]
- Maggi N., Pallanza R., Sensi P. New derivatives of rifamycin SV. Antimicrob Agents Chemother (Bethesda) 1965;5:765–769. [PubMed] [Google Scholar]
- Nagaya A., Pogo B. G., Dales S. Biogenesis of vaccinia: separation of early stages from maturation by means of rifampicin. Virology. 1970 Apr;40(4):1039–1051. doi: 10.1016/0042-6822(70)90150-9. [DOI] [PubMed] [Google Scholar]
- Subak-Sharpe J. H., Timbury M. C., Williams J. F. Rifampicin inhibits the growth of some mammalian viruses. Nature. 1969 Apr 26;222(5191):341–345. doi: 10.1038/222341a0. [DOI] [PubMed] [Google Scholar]