Abstract
An experimental system is described for the detection and quantitative estimation of type A botulinum toxin by electroimmunodiffusion. The method is shown to be rapid, specific, and quantitative. As little as 14 mouse LD50 per 0.1 ml of type A toxin was detected within 2 hr. When applied to experimentally contaminated foods such as canned tuna, pumpkin, spinach, green beans, and sausage, the technique detected botulinum toxin rapidly and identified it as to type and quantity. A specific rabbit type A antitoxin was produced for this in vitro system since the equine antitoxin (Center for Disease Control) tested in this experiment was found to be unsuitable.
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- BOOTHROYD M., GEORGALA D. L. IMMUNOFLUORESCENT IDENTIFICATION OF CLOSTRIDIUM BOTULINUM. Nature. 1964 May 2;202:515–516. doi: 10.1038/202515a0. [DOI] [PubMed] [Google Scholar]
- CROWLE A. J. A simplified micro double-diffusion agar precipitin technique. J Lab Clin Med. 1958 Nov;52(5):784–787. [PubMed] [Google Scholar]
- DUFF J. T., WRIGHT G. G., KLERER J., MOORE D. E., BIBLER R. H. Studies on immunity to toxins of Clostridium botulinum. I. A simplified procedure for isolation of type A toxin. J Bacteriol. 1957 Jan;73(1):42–47. doi: 10.1128/jb.73.1.42-47.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson H. M., Brenner K., Angelotti R., Hall H. E. Serological studies of types A, B, and E botulinal toxins by passive hemagglutination and bentonite flocculation. J Bacteriol. 1966 Mar;91(3):967–974. doi: 10.1128/jb.91.3.967-974.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lopez M., Tsu T., Hyslop N. E., Jr Studies of electroimmunodiffusion: immunochemical quantitation of proteins in dilute solutions. Immunochemistry. 1969 Jul;6(4):513–526. doi: 10.1016/0019-2791(69)90191-8. [DOI] [PubMed] [Google Scholar]
- Midura T., Taclindo C., Jr, Nygaard G. S., Bodily H. L., Wood R. M. Use of immunofluorescence and animal tests to detect growth and toxin production by Clostridum botulinum type E in food. Appl Microbiol. 1968 Jan;16(1):102–105. doi: 10.1128/am.16.1.102-105.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vermilyea B. L., Walker H. W., Ayres J. C. Detection of botulinal toxins by immunodiffusion. Appl Microbiol. 1968 Jan;16(1):21–24. doi: 10.1128/am.16.1.21-24.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]