Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1971 Sep;4(3):189–193. doi: 10.1128/iai.4.3.189-193.1971

Clinical Application of Immunofluorescence III. Identification of Lancefield Group B Streptococci

Thomas B Smith 1
PMCID: PMC416286  PMID: 4949487

Abstract

The identification of Lancefield group B streptococci with immunofluorescence was studied after significant differences between the precipitin and immunofluorescent results were noted. It was found that strains of group B streptococci, unlike groups A, C, and G streptococci, do not reliably react with a fluorescein isothiocyanate-labeled conjugate that contains antibodies mainly against C polysaccharide. It was assumed that the difficulty probably resulted from a blocking of the antigen-antibody reaction between the group antigen and its antibody by the S antigen located in the capsule. On the basis of this assumption, a conjugate was prepared that contained antibodies against the various S antigens of group B and it was found to be effective.

Full text

PDF
189

Images in this article

192Image
on p.192
192Image
on p.192
192Image
on p.192
192Image
on p.192

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braunstein H., Tucker E. B., Gibson B. C. Identification and significance of Streptococcus agalactiae (Lancefield group B). Am J Clin Pathol. 1969 Feb;51(2):207–213. doi: 10.1093/ajcp/51.2.207. [DOI] [PubMed] [Google Scholar]
  2. KARAKAWA W. W., BORMAN E. K., MCFARLAND C. R. TYPING OF GROUP A STREPTOCOCCI BY IMMUNOFLUORESCENCE. I. PREPARATION AND PROPERTIES OF TYPE I FLUORESCEIN-LABELED ANTIBODY. J Bacteriol. 1964 Jun;87:1377–1382. doi: 10.1128/jb.87.6.1377-1382.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kubin V., Jelínková J., Franek J. The use of fluorescent antibodiein e identification of streptococci. 3. Streptococcus B group typing by means of the precipitation and immunofluorescence methods. J Hyg Epidemiol Microbiol Immunol. 1969;13(3):382–388. [PubMed] [Google Scholar]
  4. Lancefield R. C., Freimer E. H. Type-specific polysaccharide antigens of group B streptococci. J Hyg (Lond) 1966 Jun;64(2):191–203. doi: 10.1017/s0022172400040456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. NASTUK W. L., PLESCIA O. J., OSSERMAN K. E. Changes in serum complement activity in patients with myasthenia gravis. Proc Soc Exp Biol Med. 1960 Oct;105:177–184. doi: 10.3181/00379727-105-26050. [DOI] [PubMed] [Google Scholar]
  6. RANTZ L. A., RANDALL E. Use of autoclaved extracts of hemolytic streptococci for serological grouping. Stanford Med Bull. 1955 May;13(2):290–291. [PubMed] [Google Scholar]
  7. SMITH T. B. CLINICAL APPLICATION OF IMMUNOFLUORESCENCE. I. GROUPING BETA-HEMOLYTIC STREPTOCOCCI. J Bacteriol. 1965 Jan;89:198–204. doi: 10.1128/jb.89.1.198-204.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Smith T. B., Heymer B., Haferkamp O., Böckeler M. Uber die Verbreitung beta-hämolytischer Streptokokken im Mund-Rachenbereich bei Bonner Schulkindern. Z Med Mikrobiol Immunol. 1966;152(4):288–299. [PubMed] [Google Scholar]
  9. Smith T. B., Heymer B., Haferkamp O. Klinische Anwendung der Immunofluorescenz. II. Präparation der für das Immunofluorescenzverfahren erforderlichen Reagentien und Nachweis von im Gewebe lokalisierten Bakterien. Z Med Mikrobiol Immunol. 1966;152(4):362–377. [PubMed] [Google Scholar]
  10. Smith T. B. Serotypen Beta-hämolysierender Streptokokken der Gruppe A in West-Deutschland. Klin Wochenschr. 1970 Aug 15;48(16):1014–1014. doi: 10.1007/BF01484412. [DOI] [PubMed] [Google Scholar]
  11. Wood B. T., Thompson S. H., Goldstein G. Fluorescent antibody staining. 3. Preparation of fluorescein-isothiocyanate-labeled antibodies. J Immunol. 1965 Aug;95(2):225–229. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES