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. 1971 Oct;4(4):503–505. doi: 10.1128/iai.4.4.503-505.1971

Separation of Two Hemolysins from Aeromonas hydrophila by Isoelectric Focusing

Bengt Wretlind 1,2, Roland Möllby 1,2, Torkel Wadström 1,2,1
PMCID: PMC416338  PMID: 5154892

Abstract

Two hemolytic proteins (hemolysin I and II) with isoelectric points of 5.5 and 4.3 were purified by isoelectric focusing from the culture supernatant fluid of Aeromonas hydrophila. The purified hemolysins were unstable after dialysis and upon heating at 37 C and were inactivated by proteolytic enzymes. The two hemolysins were lethal for mice in a dose of 10 μg (hemolysin I) and 3 μg (hemolysin II) and showed similar hemolytic spectra on red cells of different species. Both were also cytotoxic for HeLa cells and human fibroblasts and gave dermonecrosis in rabbits.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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