Figure 1.

Here illustrates the global, discovery (A) and targeted qualification (B) bottom-up proteomics workflows in which relative and absolute quantification was performed on a set of longitudinal plasma samples of a healthy bird and late-stage EOC bird. The discovery work (A) was previously performed and the SpC trend of a possible EOC candidate marker is representative from Hawkridge et al.²⁸ The targeted approach SRM-based assay (B) rapidly validates selected proteins from the global discovery evaluation and furthers the progression of the potentiality of the candidate biomarker such as demonstrated here.