Figure 4.

Mouse and human islet Ca²⁺ influx is limited by TASK-1 activity. A, Mouse islet intracellular Ca²⁺ response switching from 2mM glucose to 14mM glucose (black bar) followed by the addition of 250mM A1899 (gray bar). B, Mouse islet Ca²⁺ response switching from 2mM glucose to 14mM glucose with 100μM tolbutamide (black bar) followed by the addition of 250nM A1899 (gray bar). C, Area under the Ca²⁺ influx curve of the mouse islets for 5 minutes in 14mM glucose with 100μM tolbutamide (black bar) and for 5 minutes immediately after addition of 250nM A1899 (gray bar; n = 84, ±SEM; ***, P < .001). D, Human islet Ca²⁺ response switching from 2mM glucose to 14mM glucose with 100μM tolbutamide and 25mM TEA (black bar) followed by the addition of 250nM A1899 (gray bar). E, Human islet Ca²⁺ response switching from 2mM glucose to 14mM glucose with 100μM tolbutamide (black bar) followed by the addition of 250nM A1899 (gray bar). F, Area under the Ca²⁺ influx curve of human islets for 5 minutes in 14mM glucose with 100μM tolbutamide (black bar) and for 5 minutes immediately after addition of 250nM A1899 (gray bar; n = 50, ±SEM; *, P < .05).