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. 2014 Jun 15;5(14):5392–5402. doi: 10.18632/oncotarget.2107

Figure 1. FOXO1 is downregulated in PMBL.

Figure 1

(A) High-magnification images show differential FOXO1 expression in malignant cells of PMBL (original magnification ×200). Objective: Plan-Neofluar 40×/0.75 NA. (B) FOXO1 mRNA expression is downregulated in PMBL cases. RNA was isolated from CD19+ tonsillar cells and from frozen PMBL cases, and FOXO1 expression was measured by Q-RT-PCR and analyzed by delta Ct method. Here and in all other Q-RT-PCR experiments the house keeping gene RPL13A was used as reference and the data are shown as mean±SD of at least 2 independent Q-RT-PCR runs that included 3 technical replicates each.