Table 4. Data summary from sample dilution dissociation assessment.

Molar Ratio of Drug: Ligand	DF 10 (ng/mL)	DF 25 (ng/mL)	DF 50 (ng/mL)	DF 75 (ng/mL)	DF 100 (ng/mL)	Mean ng/mL PCSK9 of Test Series	STD	CV
0	149	163	173	163	174	164.4	10.090	6%
0.174	103	124	130	131	125	122.6	11.371	9%
0.347	72.9	84.1	82.7	94.5	79.8	82.8	7.836	9%
%Bias from Mean ng/mL Concentration of PCSK9
Molar Ratio of Drug: Ligand	DF 10	DF 25	DF 50	DF 75	DF 100
0	-9	-1	5	-1	6
0.174	-16	1	6	7	2
0.347	-12	2	0	14	-4

DF, Dilution factor; %Bias calculated as: [(observed -mean ng/mL PCSK9 of test series)/ mean ng/mL PCSK9 of test series)] x 100.