Figure 2. 4EGI-1 promotes breast CSC differentiation.

(a) Representative fluorescence-activated cell sorting (FACS) images. CD44^high/CD24^low population cells and CD44^low/CD24^high population cells with DMSO, 4μM 4EGI-N(410E), 4μM[E]-4EGI-1, or 4μM[Z]-4EGI-1 treatment for three days were shown. Both 4EGI-1 isomers promote breast CSC differentiation. (b) Statistical analyses of 4EGI-1 effects on CD44^high/CD24^low population cell percentage of breast CSCs. Average CD44^high/CD24^low percentages were shown (mean ± SD, t-test, two-tailed). *: p< 0.1; **: p< 0.05; ***: p< 0.01.