Figure 7.

Dynamic dimethylarginine binding of Tudor domains. Comparisons of experimental sPREs with sPREs back-calculated from the structures determined by NMR spectroscopy and X-ray analysis are shown. The localisation of the dimethylarginine methyl groups in the structures determined by NMR spectroscopy and X-ray analysis is highlighted. sPREs were obtained from the ¹H R₁ values measured by using a saturation-recovery scheme preceding an F2/F1 ¹⁵N/¹³C-filtered 2D NOESY spectra recorded on samples containing unlabeled ligand and five molar excess of ¹⁵N/¹³C-labeled SMN Tudor with a mixing time of 100 ms. Recovery times were between 0.01 and 4.0 s at concentrations of 0, 0.5, 1, 2, 3 and 5 mm of the soluble paramagnetic agent Gd(DTPA-BMA). Back-calculation and data analysis was carried out according to a procedure reported by Madl et al.[4b]